Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 124023..124666 Replicon plasmid p1
Accession NZ_CP104517
Organism Escherichia coli strain E4

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag N5O79_RS24930 Protein ID WP_001034044.1
Coordinates 124250..124666 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag N5O79_RS24925 Protein ID WP_001261286.1
Coordinates 124023..124253 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O79_RS24910 (N5O79_24910) 119071..121863 - 2793 WP_032492269.1 hypothetical protein -
N5O79_RS24915 (N5O79_24915) 122125..122822 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
N5O79_RS24920 (N5O79_24920) 122833..123642 - 810 WP_261307172.1 hypothetical protein -
N5O79_RS24925 (N5O79_24925) 124023..124253 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N5O79_RS24930 (N5O79_24930) 124250..124666 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
N5O79_RS24935 (N5O79_24935) 124741..126306 + 1566 WP_261307173.1 AAA family ATPase -
N5O79_RS24940 (N5O79_24940) 126291..127313 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucB / iucA 1..146375 146375
- inside IScluster/Tn - - 122319..128070 5751


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T258235 WP_001034044.1 NZ_CP104517:124250-124666 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT258235 WP_001261286.1 NZ_CP104517:124023-124253 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References