Detailed information of TA system TA025823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1558851..1559491
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: TBSG_RS07495
• Protein ID: WP_003412970.1
• Coordinates: 1559072..1559491 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: TBSG_RS07490
• Protein ID: WP_003412975.1
• Coordinates: 1558851..1559075 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS07470	1554468..1555031	+	564	WP_003412989.1	elongation factor P	-
TBSG_RS07475	1555034..1555504	+	471	WP_003899364.1	transcription antitermination factor NusB	-
TBSG_RS07480	1555504..1555905	+	402	WP_003412981.1	hypothetical protein	-
TBSG_RS07485	1555977..1558820	+	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
TBSG_RS07490	1558851..1559075	+	225	WP_003412975.1	antitoxin VapB39	Antitoxin
TBSG_RS07495	1559072..1559491	+	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBSG_RS07500	1559492..1560442	-	951	WP_003911916.1	Lsr2 family protein	-
TBSG_RS22285	1560501..1560746	+	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
TBSG_RS07505	1561087..1562007	+	921	WP_003412965.1	restriction endonuclease	-
TBSG_RS07510	1562042..1562443	-	402	WP_003412963.1	PIN domain-containing protein	-
TBSG_RS07515	1562440..1562667	-	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
TBSG_RS07520	1563184..1563906	+	723	WP_003412957.1	DUF1906 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T25823 WP_003412970.1 NC_016768:1559072-1559491 [Mycobacterium tuberculosis KZN 4207]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T25823 NC_016768:1559072-1559491 [Mycobacterium tuberculosis KZN 4207]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT25823 WP_003412975.1 NC_016768:1558851-1559075 [Mycobacterium tuberculosis KZN 4207]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT25823 NC_016768:1558851-1559075 [Mycobacterium tuberculosis KZN 4207]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09