Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2665644..2666141 Replicon chromosome
Accession NZ_CP104516
Organism Escherichia coli strain E4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5O79_RS13525 Protein ID WP_001173343.1
Coordinates 2665644..2665817 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N5O79_RS13530 Protein ID WP_000398619.1
Coordinates 2665845..2666141 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O79_RS13515 (2662314) 2662314..2663771 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
N5O79_RS13520 (2664008) 2664008..2665525 + 1518 WP_001295090.1 lysine--tRNA ligase -
N5O79_RS13525 (2665644) 2665644..2665817 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5O79_RS13530 (2665845) 2665845..2666141 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5O79_RS13535 (2666368) 2666368..2666640 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
N5O79_RS13540 (2666652) 2666652..2666882 - 231 WP_001070602.1 4Fe-4S mono-cluster protein YjdI -
N5O79_RS13545 (2667063) 2667063..2668694 + 1632 WP_001216477.1 sensor histidine kinase -
N5O79_RS13550 (2668691) 2668691..2669410 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258226 WP_001173343.1 NZ_CP104516:c2665817-2665644 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258226 WP_000398619.1 NZ_CP104516:c2666141-2665845 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References