Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1780072..1780909 Replicon chromosome
Accession NZ_CP104516
Organism Escherichia coli strain E4

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag N5O79_RS09155 Protein ID WP_000227786.1
Coordinates 1780367..1780909 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag N5O79_RS09150 Protein ID WP_001297137.1
Coordinates 1780072..1780383 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O79_RS09125 (1775092) 1775092..1776039 + 948 WP_261307137.1 cytochrome o ubiquinol oxidase subunit II -
N5O79_RS09130 (1776061) 1776061..1778052 + 1992 WP_261307138.1 cytochrome o ubiquinol oxidase subunit I -
N5O79_RS09135 (1778042) 1778042..1778656 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N5O79_RS09140 (1778656) 1778656..1778985 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N5O79_RS09145 (1778997) 1778997..1779887 + 891 WP_000971336.1 heme o synthase -
N5O79_RS09150 (1780072) 1780072..1780383 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
N5O79_RS09155 (1780367) 1780367..1780909 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
N5O79_RS09160 (1780965) 1780965..1781900 - 936 WP_001297127.1 tetratricopeptide repeat protein -
N5O79_RS09165 (1782317) 1782317..1783681 + 1365 WP_001000978.1 MFS transporter -
N5O79_RS09170 (1783809) 1783809..1784300 - 492 WP_001138904.1 nucleotide binding protein YajQ -
N5O79_RS09175 (1784468) 1784468..1785379 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T258220 WP_000227786.1 NZ_CP104516:1780367-1780909 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT258220 WP_001297137.1 NZ_CP104516:1780072-1780383 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References