Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1544521..1545152 Replicon chromosome
Accession NC_016768
Organism Mycobacterium tuberculosis KZN 4207

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag TBSG_RS07400 Protein ID WP_003413174.1
Coordinates 1544521..1544898 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag TBSG_RS07405 Protein ID WP_003413167.1
Coordinates 1544895..1545152 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBSG_RS07365 1539988..1540500 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
TBSG_RS07370 1540493..1541746 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
TBSG_RS07375 1541743..1542552 + 810 WP_003413193.1 shikimate dehydrogenase -
TBSG_RS07380 1542564..1542983 + 420 WP_003413190.1 A24 family peptidase -
TBSG_RS07385 1543394..1543639 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
TBSG_RS07390 1543636..1544031 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
TBSG_RS07395 1544131..1544505 + 375 WP_003413177.1 hypothetical protein -
TBSG_RS07400 1544521..1544898 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
TBSG_RS07405 1544895..1545152 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
TBSG_RS07410 1545191..1545604 - 414 WP_003413164.1 PIN domain nuclease -
TBSG_RS07415 1545697..1545975 - 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
TBSG_RS07420 1545972..1546634 - 663 WP_003900848.1 hypothetical protein -
TBSG_RS07425 1546631..1547290 - 660 WP_003900846.1 LppA family lipoprotein -
TBSG_RS07430 1547417..1548628 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -
TBSG_RS07435 1548924..1549238 - 315 WP_009937839.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T25821 WP_003413174.1 NC_016768:c1544898-1544521 [Mycobacterium tuberculosis KZN 4207]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T25821 NC_016768:c1544898-1544521 [Mycobacterium tuberculosis KZN 4207]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT25821 WP_003413167.1 NC_016768:c1545152-1544895 [Mycobacterium tuberculosis KZN 4207]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT25821 NC_016768:c1545152-1544895 [Mycobacterium tuberculosis KZN 4207]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References