Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4001465..4002119 Replicon chromosome
Accession NZ_CP104509
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag N5O74_RS20175 Protein ID WP_000244777.1
Coordinates 4001465..4001872 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N5O74_RS20180 Protein ID WP_000354046.1
Coordinates 4001853..4002119 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O74_RS20155 (3997422) 3997422..3999155 - 1734 WP_134235353.1 single-stranded-DNA-specific exonuclease RecJ -
N5O74_RS20160 (3999161) 3999161..3999871 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N5O74_RS20165 (3999896) 3999896..4000792 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
N5O74_RS20170 (4000904) 4000904..4001425 + 522 WP_001055874.1 flavodoxin FldB -
N5O74_RS20175 (4001465) 4001465..4001872 - 408 WP_000244777.1 protein YgfX Toxin
N5O74_RS20180 (4001853) 4001853..4002119 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N5O74_RS20185 (4002362) 4002362..4003342 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
N5O74_RS20190 (4003538) 4003538..4004197 - 660 WP_000250274.1 hemolysin III family protein -
N5O74_RS20195 (4004361) 4004361..4004672 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
N5O74_RS20200 (4004717) 4004717..4006150 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
N5O74_RS20205 (4006207) 4006207..4006950 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T258199 WP_000244777.1 NZ_CP104509:c4001872-4001465 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT258199 WP_000354046.1 NZ_CP104509:c4002119-4001853 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References