Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 258647..260270 Replicon plasmid p1
Accession NZ_CP104504
Organism Escherichia coli strain E1

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag N5O81_RS25145 Protein ID WP_000088046.1
Coordinates 258945..260270 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag N5O81_RS25140 Protein ID WP_024136327.1
Coordinates 258647..258940 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O81_RS25110 (254519) 254519..255760 + 1242 WP_001053338.1 VWA domain-containing protein -
N5O81_RS25115 (255851) 255851..255982 + 132 WP_000374059.1 hypothetical protein -
N5O81_RS25120 (256323) 256323..256604 - 282 WP_000077926.1 hypothetical protein -
N5O81_RS25125 (256654) 256654..256845 - 192 WP_000398480.1 hypothetical protein -
N5O81_RS25130 (256937) 256937..257278 - 342 WP_001151575.1 hypothetical protein -
N5O81_RS25135 (257651) 257651..258043 + 393 WP_000341066.1 hypothetical protein -
N5O81_RS25140 (258647) 258647..258940 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
N5O81_RS25145 (258945) 258945..260270 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
N5O81_RS25150 (260331) 260331..260537 + 207 WP_000134171.1 hypothetical protein -
N5O81_RS25155 (260639) 260639..260929 + 291 WP_000985910.1 hypothetical protein -
N5O81_RS25160 (260971) 260971..261807 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
N5O81_RS25165 (261807) 261807..262610 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
N5O81_RS25170 (262676) 262676..263245 - 570 WP_050545069.1 recombinase family protein -
N5O81_RS25175 (263290) 263290..263727 - 438 Protein_267 ATP-binding protein -
N5O81_RS25180 (263781) 263781..264485 - 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aph(6)-Id / aph(3'')-Ib / aac(3)-IVa / aph(4)-Ia / sitABCD iroB / iroC / iroD / iroE / iroN 1..390109 390109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T258177 WP_000088046.1 NZ_CP104504:258945-260270 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT258177 WP_024136327.1 NZ_CP104504:258647-258940 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References