Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3860708..3861284 Replicon chromosome
Accession NZ_CP104503
Organism Escherichia coli strain E1

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag N5O81_RS18635 Protein ID WP_001350372.1
Coordinates 3860708..3860995 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag N5O81_RS18640 Protein ID WP_000063155.1
Coordinates 3860982..3861284 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O81_RS18620 (3856343) 3856343..3857674 - 1332 WP_000505211.1 restriction endonuclease subunit S -
N5O81_RS18625 (3857664) 3857664..3859283 - 1620 WP_001029741.1 class I SAM-dependent DNA methyltransferase -
N5O81_RS18630 (3859347) 3859347..3860511 - 1165 Protein_3640 restriction endonuclease -
N5O81_RS18635 (3860708) 3860708..3860995 + 288 WP_001350372.1 BrnT family toxin Toxin
N5O81_RS18640 (3860982) 3860982..3861284 + 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
N5O81_RS18645 (3861318) 3861318..3862274 - 957 WP_001350371.1 GTPase -
N5O81_RS18650 (3862285) 3862285..3862488 - 204 WP_000467859.1 YbdD/YjiX family protein -
N5O81_RS18655 (3862606) 3862606..3864756 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T258172 WP_001350372.1 NZ_CP104503:3860708-3860995 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT258172 WP_000063155.1 NZ_CP104503:3860982-3861284 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References