Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3639830..3640327 Replicon chromosome
Accession NZ_CP104503
Organism Escherichia coli strain E1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5O81_RS17630 Protein ID WP_001173343.1
Coordinates 3640154..3640327 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag N5O81_RS17625 Protein ID WP_001312331.1
Coordinates 3639830..3640126 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O81_RS17605 (3636559) 3636559..3637278 - 720 WP_000611288.1 two-component system response regulator DcuR -
N5O81_RS17610 (3637275) 3637275..3638906 - 1632 WP_001216466.1 sensor histidine kinase -
N5O81_RS17615 (3639087) 3639087..3639317 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N5O81_RS17620 (3639329) 3639329..3639601 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5O81_RS17625 (3639830) 3639830..3640126 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5O81_RS17630 (3640154) 3640154..3640327 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5O81_RS17635 (3640446) 3640446..3641963 - 1518 WP_000003805.1 lysine--tRNA ligase -
N5O81_RS17640 (3642200) 3642200..3643657 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3639830..3649181 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258171 WP_001173343.1 NZ_CP104503:3640154-3640327 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT258171 WP_001312331.1 NZ_CP104503:3639830-3640126 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References