Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1122682..1123272 Replicon chromosome
Accession NZ_CP104503
Organism Escherichia coli strain E1

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag N5O81_RS05725 Protein ID WP_000373724.1
Coordinates 1122682..1123014 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag N5O81_RS05730 Protein ID WP_000288812.1
Coordinates 1123015..1123272 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O81_RS05705 (1118145) 1118145..1119407 + 1263 WP_001609141.1 integrase arm-type DNA-binding domain-containing protein -
N5O81_RS05710 (1119546) 1119546..1120004 - 459 WP_000526101.1 IS200/IS605-like element IS200C family transposase -
N5O81_RS05715 (1120956) 1120956..1121834 - 879 WP_000511773.1 integrase domain-containing protein -
N5O81_RS05720 (1122213) 1122213..1122635 + 423 WP_001164104.1 hypothetical protein -
N5O81_RS05725 (1122682) 1122682..1123014 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N5O81_RS05730 (1123015) 1123015..1123272 - 258 WP_000288812.1 antitoxin Antitoxin
N5O81_RS05735 (1123370) 1123370..1123561 + 192 WP_077561361.1 hypothetical protein -
N5O81_RS05740 (1123620) 1123620..1123826 - 207 WP_000983730.1 helix-turn-helix transcriptional regulator -
N5O81_RS05745 (1123823) 1123823..1124290 - 468 WP_000201267.1 hypothetical protein -
N5O81_RS05750 (1124523) 1124523..1125659 - 1137 WP_000420971.1 ISAs1-like element ISEc1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1106203..1153200 46997
- inside IScluster/Tn - - 1119546..1125659 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T258164 WP_000373724.1 NZ_CP104503:c1123014-1122682 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT258164 WP_000288812.1 NZ_CP104503:c1123272-1123015 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References