Detailed information of TA system TA025816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1343690..1344377
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: TBSG_RS06310
• Protein ID: WP_003414064.1
• Coordinates: 1343690..1344085 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: TBSG_RS06315
• Protein ID: WP_003414061.1
• Coordinates: 1344111..1344377 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS06280	1339587..1340324	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
TBSG_RS06285	1340480..1341280	+	801	WP_009939104.1	thymidylate synthase	-
TBSG_RS06290	1341351..1341830	+	480	WP_003414073.1	dihydrofolate reductase	-
TBSG_RS06295	1341904..1342323	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
TBSG_RS06300	1342320..1343414	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
TBSG_RS06305	1343424..1343693	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
TBSG_RS06310	1343690..1344085	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBSG_RS06315	1344111..1344377	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBSG_RS06320	1344374..1344790	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
TBSG_RS06325	1344877..1346499	+	1623	WP_003906897.1	type I restriction-modification system subunit M	-
TBSG_RS06330	1346496..1346771	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
TBSG_RS06335	1347015..1347767	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
TBSG_RS06340	1347836..1348738	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T25816 WP_003414064.1 NC_016768:1343690-1344085 [Mycobacterium tuberculosis KZN 4207]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T25816 NC_016768:1343690-1344085 [Mycobacterium tuberculosis KZN 4207]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT25816 WP_003414061.1 NC_016768:1344111-1344377 [Mycobacterium tuberculosis KZN 4207]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT25816 NC_016768:1344111-1344377 [Mycobacterium tuberculosis KZN 4207]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64