Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 638054..639642 Replicon chromosome
Accession NZ_CP104503
Organism Escherichia coli strain E1

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag N5O81_RS03260 Protein ID WP_001125432.1
Coordinates 638054..639376 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag N5O81_RS03265 Protein ID WP_001304368.1
Coordinates 639376..639642 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O81_RS03220 (633099) 633099..633341 - 243 WP_000699010.1 phage capsid protein -
N5O81_RS03225 (633545) 633545..633862 - 318 WP_000073833.1 DNA-binding protein -
N5O81_RS03230 (633874) 633874..634926 - 1053 WP_261307264.1 phage/plasmid replication protein, II/X family -
N5O81_RS03235 (635096) 635096..635278 + 183 WP_001335098.1 hypothetical protein -
N5O81_RS03240 (635310) 635310..636152 - 843 Protein_642 phage/plasmid replication protein, II/X family -
N5O81_RS03245 (636322) 636322..636504 + 183 WP_001335098.1 hypothetical protein -
N5O81_RS03250 (636605) 636605..637027 + 423 WP_042079484.1 hypothetical protein -
N5O81_RS03255 (637131) 637131..637883 + 753 WP_064181314.1 hypothetical protein -
N5O81_RS03260 (638054) 638054..639376 - 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
N5O81_RS03265 (639376) 639376..639642 - 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
N5O81_RS03270 (639950) 639950..640708 + 759 WP_001286543.1 trans-aconitate 2-methyltransferase -
N5O81_RS03275 (640712) 640712..641626 - 915 WP_001313828.1 bestrophin family protein -
N5O81_RS03280 (641823) 641823..643274 - 1452 WP_000854639.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T258159 WP_001125432.1 NZ_CP104503:c639376-638054 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT258159 WP_001304368.1 NZ_CP104503:c639642-639376 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References