Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 213930..215553 Replicon plasmid p1
Accession NZ_CP104501
Organism Escherichia coli strain E9

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag N5O75_RS26265 Protein ID WP_000088046.1
Coordinates 214228..215553 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag N5O75_RS26260 Protein ID WP_024136327.1
Coordinates 213930..214223 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O75_RS26230 (N5O75_26235) 209802..211043 + 1242 WP_001053338.1 VWA domain-containing protein -
N5O75_RS26235 (N5O75_26240) 211134..211265 + 132 WP_000374059.1 hypothetical protein -
N5O75_RS26240 (N5O75_26245) 211606..211887 - 282 WP_000077926.1 hypothetical protein -
N5O75_RS26245 (N5O75_26250) 211937..212128 - 192 WP_000398480.1 hypothetical protein -
N5O75_RS26250 (N5O75_26255) 212220..212561 - 342 WP_001151575.1 hypothetical protein -
N5O75_RS26255 (N5O75_26260) 212934..213326 + 393 WP_000341066.1 hypothetical protein -
N5O75_RS26260 (N5O75_26265) 213930..214223 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
N5O75_RS26265 (N5O75_26270) 214228..215553 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
N5O75_RS26270 (N5O75_26275) 215614..215820 + 207 WP_000134171.1 hypothetical protein -
N5O75_RS26275 (N5O75_26280) 215922..216332 + 411 WP_000985911.1 hypothetical protein -
N5O75_RS26280 (N5O75_26285) 216345..217160 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
N5O75_RS26285 (N5O75_26290) 217307..218143 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
N5O75_RS26290 (N5O75_26295) 218143..218946 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
N5O75_RS26295 (N5O75_26300) 219251..220255 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib etpB 1..281548 281548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T258157 WP_000088046.1 NZ_CP104501:214228-215553 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT258157 WP_024136327.1 NZ_CP104501:213930-214223 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References