Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 84552..85177 Replicon plasmid p1
Accession NZ_CP104501
Organism Escherichia coli strain E9

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag N5O75_RS25530 Protein ID WP_000911314.1
Coordinates 84779..85177 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID L4J1D2
Locus tag N5O75_RS25525 Protein ID WP_000450532.1
Coordinates 84552..84779 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O75_RS25525 (N5O75_25530) 84552..84779 + 228 WP_000450532.1 toxin-antitoxin system antitoxin VapB Antitoxin
N5O75_RS25530 (N5O75_25535) 84779..85177 + 399 WP_000911314.1 type II toxin-antitoxin system VapC family toxin Toxin
N5O75_RS25535 (N5O75_25540) 85186..87420 - 2235 WP_089624951.1 type IV conjugative transfer system coupling protein TraD -
N5O75_RS25540 (N5O75_25545) 87673..88404 - 732 WP_000850423.1 conjugal transfer complement resistance protein TraT -
N5O75_RS25545 (N5O75_25550) 88418..88927 - 510 WP_086589848.1 conjugal transfer entry exclusion protein TraS -
N5O75_RS25550 (N5O75_25555) 88924..89328 - 405 Protein_67 conjugal transfer protein TraG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib etpB 1..281548 281548
- flank IS/Tn - - 89434..90642 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14814.12 Da        Isoelectric Point: 7.8604

>T258154 WP_000911314.1 NZ_CP104501:84779-85177 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8512.55 Da        Isoelectric Point: 4.5358

>AT258154 WP_000450532.1 NZ_CP104501:84552-84779 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHF5

References