Detailed information of TA system TA025815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1302864..1303434
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: TBSG_RS06085
• Protein ID: WP_003414166.1
• Coordinates: 1303078..1303434 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: TBSG_RS06080
• Protein ID: WP_003901465.1
• Coordinates: 1302864..1303094 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS06040	1297882..1298193	-	312	WP_003414190.1	hypothetical protein	-
TBSG_RS06045	1298298..1298555	-	258	WP_003899489.1	hypothetical protein	-
TBSG_RS06050	1298789..1300078	-	1290	Protein_1185	transposase family protein	-
TBSG_RS06055	1300142..1300333	-	192	WP_003414184.1	hypothetical protein	-
TBSG_RS06060	1300330..1300734	-	405	WP_009938577.1	hypothetical protein	-
TBSG_RS22065	1300882..1301136	+	255	WP_003917684.1	hypothetical protein	-
TBSG_RS22070	1301312..1301587	-	276	WP_003911993.1	DUF1778 domain-containing protein	-
TBSG_RS06075	1301778..1302821	+	1044	WP_003414172.1	DUF2293 domain-containing protein	-
TBSG_RS06080	1302864..1303094	+	231	WP_003901465.1	antitoxin MazE	Antitoxin
TBSG_RS06085	1303078..1303434	+	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
TBSG_RS06090	1303536..1305185	-	1650	WP_003899485.1	CocE/NonD family hydrolase	-
TBSG_RS06095	1305204..1305791	-	588	WP_003914429.1	DUF3558 family protein	-
TBSG_RS06100	1305964..1306290	+	327	WP_003414157.1	hypothetical protein	-
TBSG_RS06105	1306294..1307982	+	1689	WP_003414155.1	alpha/beta hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1292225..1331324	39099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T25815 WP_003414166.1 NC_016768:1303078-1303434 [Mycobacterium tuberculosis KZN 4207]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T25815 NC_016768:1303078-1303434 [Mycobacterium tuberculosis KZN 4207]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT25815 WP_003901465.1 NC_016768:1302864-1303094 [Mycobacterium tuberculosis KZN 4207]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT25815 NC_016768:1302864-1303094 [Mycobacterium tuberculosis KZN 4207]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3