258146

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3823143..3823980 Replicon chromosome
Accession NZ_CP104500
Organism Escherichia coli strain E9

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag N5O75_RS19140 Protein ID WP_000227784.1
Coordinates 3823438..3823980 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag N5O75_RS19135 Protein ID WP_001297137.1
Coordinates 3823143..3823454 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O75_RS19110 (3818163) 3818163..3819110 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
N5O75_RS19115 (3819132) 3819132..3821123 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
N5O75_RS19120 (3821113) 3821113..3821727 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N5O75_RS19125 (3821727) 3821727..3822056 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N5O75_RS19130 (3822068) 3822068..3822958 + 891 WP_000971336.1 heme o synthase -
N5O75_RS19135 (3823143) 3823143..3823454 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
N5O75_RS19140 (3823438) 3823438..3823980 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
N5O75_RS19145 (3824036) 3824036..3824971 - 936 WP_001297127.1 tetratricopeptide repeat protein -
N5O75_RS19150 (3825379) 3825379..3826743 + 1365 WP_001000978.1 MFS transporter -
N5O75_RS19155 (3826871) 3826871..3827362 - 492 WP_001138904.1 nucleotide binding protein YajQ -
N5O75_RS19160 (3827530) 3827530..3828441 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T258146 WP_000227784.1 NZ_CP104500:3823438-3823980 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT258146 WP_001297137.1 NZ_CP104500:3823143-3823454 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References