Detailed information of TA system TA025813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 1276547..1277151
• Replicon: chromosome
• Accession: NC_016768
• Organism: Mycobacterium tuberculosis KZN 4207

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: TBSG_RS05935
• Protein ID: WP_003414492.1
• Coordinates: 1276759..1277151 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: TBSG_RS05930
• Protein ID: WP_003414495.1
• Coordinates: 1276547..1276762 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBSG_RS05905	1271549..1272460	+	912	WP_003414505.1	sugar ABC transporter permease	-
TBSG_RS05910	1272457..1273284	+	828	WP_003414504.1	carbohydrate ABC transporter permease	-
TBSG_RS05915	1273287..1274597	+	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
TBSG_RS05920	1274590..1275672	+	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
TBSG_RS05925	1275751..1276500	-	750	WP_003414497.1	enoyl-CoA hydratase	-
TBSG_RS05930	1276547..1276762	+	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TBSG_RS05935	1276759..1277151	+	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBSG_RS05940	1277172..1277441	+	270	WP_003414489.1	DUF2277 family protein	-
TBSG_RS05945	1277438..1277983	+	546	WP_009939112.1	DUF1802 family protein	-
TBSG_RS05950	1278288..1279175	+	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
TBSG_RS05955	1279178..1280062	+	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
TBSG_RS05960	1280334..1280879	+	546	WP_003913758.1	DUF1802 family protein	-
TBSG_RS05965	1281213..1282001	+	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T25813 WP_003414492.1 NC_016768:1276759-1277151 [Mycobacterium tuberculosis KZN 4207]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T25813 NC_016768:1276759-1277151 [Mycobacterium tuberculosis KZN 4207]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT25813 WP_003414495.1 NC_016768:1276547-1276762 [Mycobacterium tuberculosis KZN 4207]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT25813 NC_016768:1276547-1276762 [Mycobacterium tuberculosis KZN 4207]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8