Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 3088909..3089542 Replicon chromosome
Accession NZ_CP104494
Organism Ralstonia pseudosolanacearum strain Cw717

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag NQS35_RS14795 Protein ID WP_016725172.1
Coordinates 3088909..3089322 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag NQS35_RS14800 Protein ID WP_011002949.1
Coordinates 3089309..3089542 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQS35_RS14775 (NQS35_14735) 3084287..3086023 + 1737 WP_016725176.1 ABC transporter substrate-binding protein -
NQS35_RS14780 (NQS35_14740) 3086185..3087681 + 1497 WP_016725175.1 glycerol kinase GlpK -
NQS35_RS14785 (NQS35_14745) 3087746..3088117 - 372 WP_016725174.1 hypothetical protein -
NQS35_RS14790 (NQS35_14750) 3088308..3088934 + 627 WP_016725173.1 DUF1415 domain-containing protein -
NQS35_RS14795 (NQS35_14755) 3088909..3089322 - 414 WP_016725172.1 PIN domain-containing protein Toxin
NQS35_RS14800 (NQS35_14760) 3089309..3089542 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
NQS35_RS14805 (NQS35_14765) 3089599..3091206 - 1608 WP_069079162.1 glucan biosynthesis protein -
NQS35_RS14810 (NQS35_14770) 3091419..3092651 + 1233 WP_021155141.1 AGE family epimerase/isomerase -
NQS35_RS14815 (NQS35_14775) 3092659..3093954 + 1296 WP_011002952.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T258125 WP_016725172.1 NZ_CP104494:c3089322-3088909 [Ralstonia pseudosolanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT258125 WP_011002949.1 NZ_CP104494:c3089542-3089309 [Ralstonia pseudosolanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References