258008

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4032780..4033617 Replicon chromosome
Accession NZ_CP104472
Organism Escherichia coli strain UC4224

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag N4S17_RS19385 Protein ID WP_000227784.1
Coordinates 4032780..4033322 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag N4S17_RS19390 Protein ID WP_001297137.1
Coordinates 4033306..4033617 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S17_RS19365 (4028319) 4028319..4029230 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
N4S17_RS19370 (4029398) 4029398..4029889 + 492 WP_001138904.1 nucleotide binding protein YajQ -
N4S17_RS19375 (4030017) 4030017..4031381 - 1365 WP_028985265.1 MFS transporter -
N4S17_RS19380 (4031789) 4031789..4032724 + 936 WP_001368479.1 tetratricopeptide repeat protein -
N4S17_RS19385 (4032780) 4032780..4033322 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
N4S17_RS19390 (4033306) 4033306..4033617 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
N4S17_RS19395 (4033802) 4033802..4034692 - 891 WP_000971336.1 heme o synthase -
N4S17_RS19400 (4034704) 4034704..4035033 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N4S17_RS19405 (4035033) 4035033..4035647 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N4S17_RS19410 (4035637) 4035637..4037628 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
N4S17_RS19415 (4037650) 4037650..4038597 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T258008 WP_000227784.1 NZ_CP104472:c4033322-4032780 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT258008 WP_001297137.1 NZ_CP104472:c4033617-4033306 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References