Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2500016..2500602 Replicon chromosome
Accession NZ_CP104472
Organism Escherichia coli strain UC4224

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag N4S17_RS12245 Protein ID WP_042014162.1
Coordinates 2500372..2500602 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag N4S17_RS12240 Protein ID WP_000593555.1
Coordinates 2500016..2500375 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S17_RS12215 (2496148) 2496148..2497092 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
N4S17_RS12220 (2497089) 2497089..2497922 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
N4S17_RS12225 (2497922) 2497922..2498686 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
N4S17_RS12230 (2498683) 2498683..2499489 + 807 WP_062865943.1 nickel import ATP-binding protein NikE -
N4S17_RS12235 (2499495) 2499495..2499896 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
N4S17_RS12240 (2500016) 2500016..2500375 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N4S17_RS12245 (2500372) 2500372..2500602 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
N4S17_RS12250 (2500706) 2500706..2501830 - 1125 WP_001314210.1 ABC transporter permease -
N4S17_RS12255 (2501830) 2501830..2504565 - 2736 WP_198458796.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T258001 WP_042014162.1 NZ_CP104472:c2500602-2500372 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT258001 WP_000593555.1 NZ_CP104472:c2500375-2500016 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References