Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1618210..1618793 Replicon chromosome
Accession NZ_CP104472
Organism Escherichia coli strain UC4224

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag N4S17_RS07890 Protein ID WP_000254745.1
Coordinates 1618210..1618545 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag N4S17_RS07895 Protein ID WP_000581937.1
Coordinates 1618545..1618793 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S17_RS07875 (1614097) 1614097..1615395 - 1299 WP_000036723.1 phosphopyruvate hydratase -
N4S17_RS07880 (1615483) 1615483..1617120 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
N4S17_RS07885 (1617348) 1617348..1618139 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
N4S17_RS07890 (1618210) 1618210..1618545 - 336 WP_000254745.1 endoribonuclease MazF Toxin
N4S17_RS07895 (1618545) 1618545..1618793 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
N4S17_RS07900 (1618871) 1618871..1621105 - 2235 WP_000226815.1 GTP pyrophosphokinase -
N4S17_RS07905 (1621153) 1621153..1622454 - 1302 WP_021542211.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T257996 WP_000254745.1 NZ_CP104472:c1618545-1618210 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT257996 WP_000581937.1 NZ_CP104472:c1618793-1618545 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References