Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4296..5919 Replicon plasmid pE80014967-1
Accession NZ_CP104468
Organism Enterobacter hormaechei strain 80014967

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NYP10_RS00035 Protein ID WP_000088046.1
Coordinates 4296..5621 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NYP10_RS00040 Protein ID WP_024136327.1
Coordinates 5626..5919 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYP10_RS00005 (NYP10_00005) 280..1137 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
NYP10_RS00010 (NYP10_00010) 1153..1461 - 309 WP_001224687.1 hypothetical protein -
NYP10_RS00015 (NYP10_00015) 2010..2435 - 426 WP_001043843.1 hypothetical protein -
NYP10_RS00020 (NYP10_00020) 2689..3504 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NYP10_RS00025 (NYP10_00025) 3517..3927 - 411 WP_000985911.1 hypothetical protein -
NYP10_RS00030 (NYP10_00030) 4029..4235 - 207 WP_000134171.1 hypothetical protein -
NYP10_RS00035 (NYP10_00035) 4296..5621 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NYP10_RS00040 (NYP10_00040) 5626..5919 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NYP10_RS00045 (NYP10_00045) 6523..6915 - 393 WP_000341066.1 hypothetical protein -
NYP10_RS00050 (NYP10_00050) 7288..7629 + 342 WP_001151575.1 hypothetical protein -
NYP10_RS00055 (NYP10_00055) 7721..7912 + 192 WP_000398480.1 hypothetical protein -
NYP10_RS00060 (NYP10_00060) 7962..8243 + 282 WP_000077926.1 hypothetical protein -
NYP10_RS00065 (NYP10_00065) 8584..8715 - 132 WP_000374059.1 hypothetical protein -
NYP10_RS00070 (NYP10_00070) 8806..10047 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / tet(A) / catA1 / ant(3'')-Ia / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 htpB 1..206102 206102


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T257971 WP_000088046.1 NZ_CP104468:c5621-4296 [Enterobacter hormaechei]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT257971 WP_024136327.1 NZ_CP104468:c5919-5626 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References