Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 16571..18139 Replicon plasmid pIncA_Silv108
Accession NZ_CP104451
Organism Raoultella ornithinolytica strain MQB_Silv_108

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag N2J37_RS27975 Protein ID WP_024134513.1
Coordinates 16571..17878 (-) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag N2J37_RS27980 Protein ID WP_039272366.1
Coordinates 17888..18139 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2J37_RS27955 (N2J37_27955) 12454..12756 + 303 WP_031611417.1 hypothetical protein -
N2J37_RS27960 (N2J37_27960) 13128..14768 - 1641 WP_031611419.1 PAAR domain-containing protein -
N2J37_RS27965 (N2J37_27965) 14967..15905 + 939 WP_044596315.1 hypothetical protein -
N2J37_RS27970 (N2J37_27970) 16080..16574 - 495 WP_039272364.1 hypothetical protein -
N2J37_RS27975 (N2J37_27975) 16571..17878 - 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
N2J37_RS27980 (N2J37_27980) 17888..18139 - 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
N2J37_RS27985 (N2J37_27985) 18504..19508 - 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
N2J37_RS27990 (N2J37_27990) 19511..20074 - 564 WP_001132404.1 hypothetical protein -
N2J37_RS27995 (N2J37_27995) 20093..20374 - 282 WP_000575656.1 hypothetical protein -
N2J37_RS28000 (N2J37_28000) 20635..21237 + 603 WP_039272372.1 hypothetical protein -
N2J37_RS28005 (N2J37_28005) 21253..21705 - 453 WP_001553092.1 hypothetical protein -
N2J37_RS28010 (N2J37_28010) 21761..22486 - 726 WP_001553093.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-12 / sul1 / qacE / aac(3)-Ib-aac(6')-Ib - 1..148888 148888


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T257966 WP_024134513.1 NZ_CP104451:c17878-16571 [Raoultella ornithinolytica]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT257966 WP_039272366.1 NZ_CP104451:c18139-17888 [Raoultella ornithinolytica]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References