257945

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4350027..4350827 Replicon chromosome
Accession NZ_CP104442
Organism Escherichia coli strain HBUT-L

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag N1H43_RS21255 Protein ID WP_000342449.1
Coordinates 4350027..4350554 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag N1H43_RS21260 Protein ID WP_001277108.1
Coordinates 4350561..4350827 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1H43_RS21230 (4345102) 4345102..4345869 - 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
N1H43_RS21235 (4345866) 4345866..4347143 - 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
N1H43_RS21240 (4347140) 4347140..4348066 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
N1H43_RS21245 (4348114) 4348114..4349223 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
N1H43_RS21250 (4349647) 4349647..4350030 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
N1H43_RS21255 (4350027) 4350027..4350554 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
N1H43_RS21260 (4350561) 4350561..4350827 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
N1H43_RS21265 (4350977) 4350977..4352080 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
N1H43_RS21270 (4352351) 4352351..4353205 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
N1H43_RS21275 (4353450) 4353450..4354508 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
N1H43_RS21280 (4354501) 4354501..4355169 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T257945 WP_000342449.1 NZ_CP104442:c4350554-4350027 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT257945 WP_001277108.1 NZ_CP104442:c4350827-4350561 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References