Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3269238..3269863 Replicon chromosome
Accession NZ_CP104442
Organism Escherichia coli strain HBUT-L

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag N1H43_RS15945 Protein ID WP_000911329.1
Coordinates 3269238..3269636 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag N1H43_RS15950 Protein ID WP_000450524.1
Coordinates 3269636..3269863 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1H43_RS15925 (3265145) 3265145..3265345 + 201 WP_000383836.1 YpfN family protein -
N1H43_RS15930 (3265426) 3265426..3266124 - 699 WP_000679823.1 esterase -
N1H43_RS15935 (3266198) 3266198..3268213 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
N1H43_RS15940 (3268228) 3268228..3269091 - 864 WP_001267505.1 neutral zinc metallopeptidase -
N1H43_RS15945 (3269238) 3269238..3269636 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
N1H43_RS15950 (3269636) 3269636..3269863 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
N1H43_RS15955 (3270018) 3270018..3270731 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
N1H43_RS15960 (3270944) 3270944..3271978 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
N1H43_RS15965 (3271995) 3271995..3272873 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
N1H43_RS15970 (3273019) 3273019..3273591 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
N1H43_RS15975 (3273591) 3273591..3274061 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T257941 WP_000911329.1 NZ_CP104442:c3269636-3269238 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT257941 WP_000450524.1 NZ_CP104442:c3269863-3269636 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References