Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1111559..1112396 Replicon chromosome
Accession NZ_CP104442
Organism Escherichia coli strain HBUT-L

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag N1H43_RS05320 Protein ID WP_000227784.1
Coordinates 1111559..1112101 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag N1H43_RS05325 Protein ID WP_001297137.1
Coordinates 1112085..1112396 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1H43_RS05300 (1107109) 1107109..1108020 - 912 WP_000705849.1 2-dehydropantoate 2-reductase -
N1H43_RS05305 (1108188) 1108188..1108679 + 492 WP_001138904.1 nucleotide binding protein YajQ -
N1H43_RS05310 (1108807) 1108807..1110171 - 1365 WP_001000978.1 MFS transporter -
N1H43_RS05315 (1110579) 1110579..1111503 + 925 Protein_1034 sel1 repeat family protein -
N1H43_RS05320 (1111559) 1111559..1112101 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
N1H43_RS05325 (1112085) 1112085..1112396 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
N1H43_RS05330 (1112581) 1112581..1113471 - 891 WP_000971336.1 heme o synthase -
N1H43_RS05335 (1113483) 1113483..1113812 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N1H43_RS05340 (1113812) 1113812..1114426 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N1H43_RS05345 (1114416) 1114416..1116407 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
N1H43_RS05350 (1116429) 1116429..1117376 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T257928 WP_000227784.1 NZ_CP104442:c1112101-1111559 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT257928 WP_001297137.1 NZ_CP104442:c1112396-1112085 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References