Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1016734..1017231 Replicon chromosome
Accession NZ_CP104442
Organism Escherichia coli strain HBUT-L

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N1H43_RS04840 Protein ID WP_001173343.1
Coordinates 1016734..1016907 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N1H43_RS04845 Protein ID WP_000398619.1
Coordinates 1016935..1017231 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1H43_RS04830 (1013404) 1013404..1014861 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
N1H43_RS04835 (1015098) 1015098..1016615 + 1518 WP_001295074.1 lysine--tRNA ligase -
N1H43_RS04840 (1016734) 1016734..1016907 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N1H43_RS04845 (1016935) 1016935..1017231 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N1H43_RS04850 (1017458) 1017458..1017730 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
N1H43_RS04855 (1017742) 1017742..1017972 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N1H43_RS04860 (1018153) 1018153..1019784 + 1632 WP_001216477.1 sensor histidine kinase -
N1H43_RS04865 (1019781) 1019781..1020500 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T257927 WP_001173343.1 NZ_CP104442:c1016907-1016734 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT257927 WP_000398619.1 NZ_CP104442:c1017231-1016935 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References