Detailed information of TA system TA025792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 5285..6063
• Replicon: plasmid pOCEGK01
• Accession: NC_016746
• Organism: Oceanimonas sp. GK1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: GU3_RS16515
• Protein ID: WP_041544020.1
• Coordinates: 5285..5779 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: H2G1Y7
• Locus tag: GU3_RS16520
• Protein ID: WP_014293675.1
• Coordinates: 5776..6063 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU3_RS17250	665..832	-	168	WP_158308474.1	hypothetical protein	-
GU3_RS16500	1357..1560	+	204	WP_014293671.1	hypothetical protein	-
GU3_RS16505	2382..3317	+	936	WP_014293672.1	integrase domain-containing protein	-
GU3_RS16510	4111..5046	-	936	WP_014293673.1	replication initiation protein	-
GU3_RS16515	5285..5779	-	495	WP_041544020.1	GNAT family N-acetyltransferase	Toxin
GU3_RS16520	5776..6063	-	288	WP_014293675.1	DUF1778 domain-containing protein	Antitoxin
GU3_RS16525	6263..6892	+	630	WP_014293676.1	AAA family ATPase	-
GU3_RS16530	6889..7113	+	225	WP_014293677.1	hypothetical protein	-
GU3_RS16535	7133..7402	+	270	WP_014293678.1	hypothetical protein	-
GU3_RS16540	7404..8135	+	732	WP_014293679.1	HNH endonuclease family protein	-
GU3_RS17200	8158..8457	+	300	WP_148265990.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8462	8462

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17767.60 Da
• Isoelectric Point: 8.5067

>T25792 WP_041544020.1 NC_016746:c5779-5285 [Oceanimonas sp. GK1]
VMLSAPELLGSHHDTRSFDCGSSNLNDWLGKRALKNQMNGGSRTYVLCDGHHVKGYYALTTGAIGPDLAVGRFRRNMPNP
IPVVLLARLAIDTSLQGKGIGRALLRDASLRVLQAADLVGIRGMIVHALDNDAKAFYERLGFDVSPIEPMTLMITVADLK
ALVE

Nucleotide

• Length: 495 bp

>T25792 NC_016746:c5779-5285 [Oceanimonas sp. GK1]
GTGATGCTGAGTGCCCCAGAACTATTGGGATCACACCACGACACCCGCTCTTTTGATTGTGGCTCAAGCAACCTGAATGA
CTGGCTGGGTAAACGTGCGCTAAAGAACCAGATGAATGGGGGCAGCCGTACTTATGTGCTGTGCGATGGGCACCATGTTA
AGGGCTACTATGCCCTGACGACCGGTGCGATTGGTCCTGATCTGGCCGTTGGCCGGTTTCGGAGAAACATGCCTAACCCT
ATTCCGGTTGTGCTGTTGGCTCGGCTGGCGATTGATACCAGTCTGCAGGGAAAAGGGATAGGTAGGGCGTTGCTGCGCGA
TGCTTCTCTTCGCGTGCTACAAGCTGCGGATCTGGTTGGTATTCGGGGCATGATTGTTCATGCATTGGACAATGACGCGA
AAGCATTCTACGAGCGTCTGGGCTTTGATGTTTCGCCAATAGAGCCAATGACGTTGATGATTACGGTTGCTGATTTGAAA
GCATTGGTTGAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10701.22 Da
• Isoelectric Point: 6.9834

>AT25792 WP_014293675.1 NC_016746:c6063-5776 [Oceanimonas sp. GK1]
MATAHVEAKRDKTLNLRIRDDERALIERAAKVCGKTRTEFIMGAVRREAADALLEQAYVSVSPEVYDAFLARLDEAPSPN
DRLRKTMSTPAPWEV

Nucleotide

• Length: 288 bp

>AT25792 NC_016746:c6063-5776 [Oceanimonas sp. GK1]
ATGGCCACTGCACATGTGGAAGCCAAACGCGATAAGACATTGAACCTGCGTATCCGTGATGATGAGCGGGCCTTGATTGA
ACGAGCAGCCAAAGTCTGCGGTAAAACACGTACGGAGTTCATCATGGGGGCTGTGCGCCGTGAGGCAGCTGATGCGTTGC
TGGAACAAGCCTATGTTTCGGTGTCCCCTGAAGTGTACGACGCATTCCTGGCACGGCTGGATGAAGCACCGAGCCCGAAT
GATCGGTTGCGTAAAACCATGTCCACACCTGCCCCCTGGGAGGTGTGA

Structures

Antitoxin

• AlphaFold DB: H2G1Y7