Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 75343..76140 Replicon plasmid pEH4236
Accession NZ_CP104406
Organism Enterobacter hormaechei subsp. steigerwaltii strain 4236

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag N5F16_RS23805 Protein ID WP_006812498.1
Coordinates 75343..75867 (-) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag N5F16_RS23810 Protein ID WP_006812497.1
Coordinates 75871..76140 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5F16_RS23780 (N5F16_23780) 73014..73802 + 789 WP_032655902.1 receptor-recognizing protein -
N5F16_RS23785 (N5F16_23785) 73877..74200 + 324 WP_000064173.1 hypothetical protein -
N5F16_RS23790 (N5F16_23790) 74214..74906 + 693 WP_006812500.1 membrane protein -
N5F16_RS23795 (N5F16_23795) 74908..75159 + 252 WP_000147960.1 hypothetical protein -
N5F16_RS23800 (N5F16_23800) 75152..75316 + 165 WP_006812499.1 hypothetical protein -
N5F16_RS23805 (N5F16_23805) 75343..75867 - 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
N5F16_RS23810 (N5F16_23810) 75871..76140 - 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
N5F16_RS23815 (N5F16_23815) 76529..77194 + 666 WP_023315962.1 AAA family ATPase -
N5F16_RS23820 (N5F16_23820) 77200..77553 + 354 WP_160859104.1 hypothetical protein -
N5F16_RS23825 (N5F16_23825) 77595..78392 - 798 WP_006812584.1 DUF2971 domain-containing protein -
N5F16_RS23830 (N5F16_23830) 78656..79381 + 726 WP_006812583.1 hypothetical protein -
N5F16_RS23835 (N5F16_23835) 79442..80782 + 1341 WP_006812582.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110892 110892


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T257917 WP_006812498.1 NZ_CP104406:c75867-75343 [Enterobacter hormaechei subsp. steigerwaltii]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT257917 WP_006812497.1 NZ_CP104406:c76140-75871 [Enterobacter hormaechei subsp. steigerwaltii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References