Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 3119800..3121583 Replicon chromosome
Accession NZ_CP104405
Organism Enterobacter hormaechei subsp. steigerwaltii strain 4236

Toxin (Protein)


Gene name hipA Uniprot ID A0A2G5A822
Locus tag N5F16_RS15045 Protein ID WP_045333653.1
Coordinates 3119800..3121149 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A2G5A843
Locus tag N5F16_RS15050 Protein ID WP_045333654.1
Coordinates 3121146..3121583 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5F16_RS15020 (N5F16_15020) 3115623..3116558 - 936 WP_133983007.1 tRNA dihydrouridine(16) synthase DusC -
N5F16_RS15025 (N5F16_15025) 3116727..3117122 + 396 WP_003859308.1 CidA/LrgA family protein -
N5F16_RS15030 (N5F16_15030) 3117119..3117814 + 696 WP_015572322.1 CidB/LrgB family autolysis modulator -
N5F16_RS15035 (N5F16_15035) 3117943..3118827 + 885 WP_133983009.1 cytidine deaminase -
N5F16_RS15040 (N5F16_15040) 3118955..3119665 + 711 WP_017383381.1 outer membrane permeability protein SanA -
N5F16_RS15045 (N5F16_15045) 3119800..3121149 + 1350 WP_045333653.1 HipA domain-containing protein Toxin
N5F16_RS15050 (N5F16_15050) 3121146..3121583 + 438 WP_045333654.1 helix-turn-helix transcriptional regulator Antitoxin
N5F16_RS15055 (N5F16_15055) 3121622..3122632 - 1011 WP_133983011.1 galactose/methyl galactoside ABC transporter permease MglC -
N5F16_RS15060 (N5F16_15060) 3122648..3124168 - 1521 WP_047365146.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
N5F16_RS15065 (N5F16_15065) 3124248..3125246 - 999 WP_047365148.1 galactose/glucose ABC transporter substrate-binding protein MglB -
N5F16_RS15070 (N5F16_15070) 3125542..3126564 - 1023 WP_045341031.1 HTH-type transcriptional regulator GalS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51197.84 Da        Isoelectric Point: 4.6562

>T257913 WP_045333653.1 NZ_CP104405:3119800-3121149 [Enterobacter hormaechei subsp. steigerwaltii]
METLTVQAYLNETWTDIALIKYPGSEKNDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRTLINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPTT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16171.61 Da        Isoelectric Point: 10.5154

>AT257913 WP_045333654.1 NZ_CP104405:3121146-3121583 [Enterobacter hormaechei subsp. steigerwaltii]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEDPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G5A822

Antitoxin

Source ID Structure
AlphaFold DB A0A2G5A843

References