Detailed information of TA system TA025790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 1670519..1671119
• Replicon: chromosome
• Accession: NC_016645
• Organism: Pyrobaculum sp. 1860

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: P186_RS09310
• Protein ID: WP_014289216.1
• Coordinates: 1670733..1671119 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: P186_RS09305
• Protein ID: WP_014289215.1
• Coordinates: 1670519..1670740 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P186_RS09270 (P186_1985)	1666162..1666719	+	558	WP_014289209.1	PaREP1 family protein	-
P186_RS09275 (P186_1986)	1667017..1667241	+	225	WP_014289210.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
P186_RS09280 (P186_1987)	1667238..1667660	+	423	WP_014289211.1	PIN domain-containing protein	-
P186_RS13865	1667922..1668092	-	171	WP_158307143.1	hypothetical protein	-
P186_RS14365	1668395..1668613	+	219	WP_237179390.1	hydrogenase iron-sulfur subunit	-
P186_RS14370	1668716..1668949	+	234	WP_237179391.1	4Fe-4S binding protein	-
P186_RS14375	1668974..1669213	+	240	WP_237179392.1	hypothetical protein	-
P186_RS09295 (P186_1992)	1669617..1670003	-	387	WP_148682925.1	hypothetical protein	-
P186_RS09300 (P186_1993)	1670301..1670489	+	189	WP_014289214.1	hypothetical protein	-
P186_RS09305 (P186_1994)	1670519..1670740	+	222	WP_014289215.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
P186_RS09310 (P186_1995)	1670733..1671119	+	387	WP_014289216.1	PIN domain-containing protein	Toxin
P186_RS13870 (P186_1996)	1671576..1671737	-	162	WP_014289217.1	hypothetical protein	-
P186_RS09315	1671997..1672176	-	180	WP_148682926.1	hypothetical protein	-
P186_RS09320 (P186_1999)	1672652..1673203	-	552	WP_014289220.1	adenylyl-sulfate kinase	-
P186_RS09325 (P186_2000)	1673200..1674174	-	975	WP_237179393.1	alkaline phosphatase family protein	-
P186_RS09330 (P186_2001)	1674300..1675589	+	1290	WP_014289222.1	nucleotide sugar dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (4-46)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14167.58 Da
• Isoelectric Point: 6.2302

>T25790 WP_014289216.1 NC_016645:1670733-1671119 [Pyrobaculum ferrireducens]
MGEAVVDTNAVIYYVVEDSPFHKEAEALLDSLDVWHLPTVVVHELVWFFKKAAPDRGIEVLRALLGYEKVVVECEDLASL
RKAASAGLAYYNDLVVILTARRLGLPLVTFDKKMARRAGAFGVPVLKP

Nucleotide

• Length: 387 bp

>T25790 NC_016645:1670733-1671119 [Pyrobaculum ferrireducens]
ATGGGTGAGGCTGTAGTCGACACGAACGCGGTGATATACTACGTGGTCGAGGATTCGCCGTTTCATAAGGAGGCCGAGGC
GCTCCTCGACTCTCTAGACGTCTGGCATCTCCCCACCGTCGTCGTCCATGAATTAGTGTGGTTTTTCAAGAAAGCGGCGC
CGGATAGGGGGATCGAGGTCTTGAGGGCCCTCCTGGGATACGAGAAGGTCGTCGTGGAGTGCGAGGACTTGGCGTCTTTG
AGGAAGGCAGCCAGCGCGGGGCTCGCCTACTACAACGACCTAGTGGTTATCCTGACGGCGAGGAGGCTGGGCCTCCCCTT
GGTCACTTTCGACAAGAAGATGGCGAGGAGGGCCGGTGCCTTTGGGGTCCCCGTGCTTAAACCTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8138.54 Da
• Isoelectric Point: 10.6625

>AT25790 WP_014289215.1 NC_016645:1670519-1670740 [Pyrobaculum ferrireducens]
MAVVKVTRNFQITIPADVRRALGIREGDRLLVEVEGDRIVIRKVAGALPRIRLGMRLTPEDIDRFVEQGAVDG

Nucleotide

• Length: 222 bp

>AT25790 NC_016645:1670519-1670740 [Pyrobaculum ferrireducens]
ATGGCGGTGGTCAAGGTGACGCGTAACTTCCAGATAACGATCCCGGCAGATGTCAGGAGGGCGTTGGGAATCCGCGAGGG
GGATCGACTGTTGGTGGAGGTCGAGGGGGATAGGATAGTGATTAGGAAGGTTGCGGGGGCGTTGCCGAGGATTAGGCTAG
GGATGAGGCTGACGCCGGAGGACATAGACAGGTTTGTAGAACAAGGCGCAGTCGATGGGTGA