Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 75343..76140 Replicon plasmid pEH4236
Accession NZ_CP104404
Organism Enterobacter hormaechei subsp. steigerwaltii strain csa13R

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag N5F14_RS23885 Protein ID WP_006812498.1
Coordinates 75343..75867 (-) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag N5F14_RS23890 Protein ID WP_006812497.1
Coordinates 75871..76140 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5F14_RS23860 (N5F14_23860) 73014..73802 + 789 WP_032655902.1 receptor-recognizing protein -
N5F14_RS23865 (N5F14_23865) 73877..74200 + 324 WP_000064173.1 hypothetical protein -
N5F14_RS23870 (N5F14_23870) 74214..74906 + 693 WP_006812500.1 membrane protein -
N5F14_RS23875 (N5F14_23875) 74908..75159 + 252 WP_000147960.1 hypothetical protein -
N5F14_RS23880 (N5F14_23880) 75152..75316 + 165 WP_006812499.1 hypothetical protein -
N5F14_RS23885 (N5F14_23885) 75343..75867 - 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
N5F14_RS23890 (N5F14_23890) 75871..76140 - 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
N5F14_RS23895 (N5F14_23895) 76522..77187 + 666 WP_023315962.1 AAA family ATPase -
N5F14_RS23900 (N5F14_23900) 77193..77546 + 354 WP_160859104.1 hypothetical protein -
N5F14_RS23905 (N5F14_23905) 77588..78385 - 798 WP_006812584.1 DUF2971 domain-containing protein -
N5F14_RS23910 (N5F14_23910) 78649..79374 + 726 WP_006812583.1 hypothetical protein -
N5F14_RS23915 (N5F14_23915) 79435..80775 + 1341 WP_006812582.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110885 110885


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T257898 WP_006812498.1 NZ_CP104404:c75867-75343 [Enterobacter hormaechei subsp. steigerwaltii]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT257898 WP_006812497.1 NZ_CP104404:c76140-75871 [Enterobacter hormaechei subsp. steigerwaltii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References