Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/FR47-DUF1778 |
| Location | 75186..75983 | Replicon | plasmid pEH4236 |
| Accession | NZ_CP104402 | ||
| Organism | Enterobacter hormaechei subsp. steigerwaltii strain colR | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | F5S3R9 |
| Locus tag | N5F15_RS23985 | Protein ID | WP_006812498.1 |
| Coordinates | 75186..75710 (-) | Length | 175 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | F5S3R8 |
| Locus tag | N5F15_RS23990 | Protein ID | WP_006812497.1 |
| Coordinates | 75714..75983 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| N5F15_RS23960 (N5F15_23960) | 72857..73645 | + | 789 | WP_032655902.1 | receptor-recognizing protein | - |
| N5F15_RS23965 (N5F15_23965) | 73720..74043 | + | 324 | WP_000064173.1 | hypothetical protein | - |
| N5F15_RS23970 (N5F15_23970) | 74057..74749 | + | 693 | WP_006812500.1 | membrane protein | - |
| N5F15_RS23975 (N5F15_23975) | 74751..75002 | + | 252 | WP_000147960.1 | hypothetical protein | - |
| N5F15_RS23980 (N5F15_23980) | 74995..75159 | + | 165 | WP_006812499.1 | hypothetical protein | - |
| N5F15_RS23985 (N5F15_23985) | 75186..75710 | - | 525 | WP_006812498.1 | GNAT family N-acetyltransferase | Toxin |
| N5F15_RS23990 (N5F15_23990) | 75714..75983 | - | 270 | WP_006812497.1 | DUF1778 domain-containing protein | Antitoxin |
| N5F15_RS23995 (N5F15_23995) | 76372..77037 | + | 666 | WP_023315962.1 | AAA family ATPase | - |
| N5F15_RS24000 (N5F15_24000) | 77043..77396 | + | 354 | WP_160859104.1 | hypothetical protein | - |
| N5F15_RS24005 (N5F15_24005) | 77438..78235 | - | 798 | WP_006812584.1 | DUF2971 domain-containing protein | - |
| N5F15_RS24010 (N5F15_24010) | 78499..79224 | + | 726 | WP_006812583.1 | hypothetical protein | - |
| N5F15_RS24015 (N5F15_24015) | 79285..80625 | + | 1341 | WP_006812582.1 | DnaB-like helicase C-terminal domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..110892 | 110892 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 175 a.a. Molecular weight: 19356.13 Da Isoelectric Point: 8.9844
>T257880 WP_006812498.1 NZ_CP104402:c75710-75186 [Enterobacter hormaechei subsp. steigerwaltii]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK
Download Length: 525 bp
>T257880 NZ_CP104402:c75710-75186 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGCCAACCTGACCATTGAGATGTTTTCAGAAGAGGCTGTATATGATTTCTCATGCTTCGACTGCGGAGAAACGTCTCT
CAATGATTTTCTTAACAATCGCCTCGCCCAGCAACACAGCGGGCGTATATTACGCGGATATCTGCTTCTGACGAGAGATC
CGATACCGAAAGTGATGGGGTTTTACACGTTGTCCGGGAGCTGCTTTGCAAGAAACACGCTTCCTTCCAACACTCAACAG
CGTAAAGTCCCCTATTCCGACGCCCCAAGCGTTACGCTCGGTCGTCTGGCAATAGACAAAAGTATCCAGCGACAAGGGTA
TGGGGAAACGCTGGTGGCACATGCGATGAAAGTGGTATACCGGGCTTCCCTGGCCGTTGGGATCTACGCTTTGTTTGTTG
ACGCCAAAAATGAGAATGCGATGCGGTTTTATAAGCAGTTAGGCTTTACCCCTCTTACGGGTGATAACGCCAATTCGCTG
TTCTATCCCACAAAAGGCATTGAGAAGTTGTTTGGTGGAAAATAA
GTGGCCAACCTGACCATTGAGATGTTTTCAGAAGAGGCTGTATATGATTTCTCATGCTTCGACTGCGGAGAAACGTCTCT
CAATGATTTTCTTAACAATCGCCTCGCCCAGCAACACAGCGGGCGTATATTACGCGGATATCTGCTTCTGACGAGAGATC
CGATACCGAAAGTGATGGGGTTTTACACGTTGTCCGGGAGCTGCTTTGCAAGAAACACGCTTCCTTCCAACACTCAACAG
CGTAAAGTCCCCTATTCCGACGCCCCAAGCGTTACGCTCGGTCGTCTGGCAATAGACAAAAGTATCCAGCGACAAGGGTA
TGGGGAAACGCTGGTGGCACATGCGATGAAAGTGGTATACCGGGCTTCCCTGGCCGTTGGGATCTACGCTTTGTTTGTTG
ACGCCAAAAATGAGAATGCGATGCGGTTTTATAAGCAGTTAGGCTTTACCCCTCTTACGGGTGATAACGCCAATTCGCTG
TTCTATCCCACAAAAGGCATTGAGAAGTTGTTTGGTGGAAAATAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10114.51 Da Isoelectric Point: 4.8483
>AT257880 WP_006812497.1 NZ_CP104402:c75983-75714 [Enterobacter hormaechei subsp. steigerwaltii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME
Download Length: 270 bp
>AT257880 NZ_CP104402:c75983-75714 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACAATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGTCGCC
TGATTCTTAGCGAAGAGTCATGGAATATAGTGATGGATGCCATCAGTAACCCGCCTGCACCGAATGATAGGCTGAAACGA
GCTGCCGAGCGTCTACAAAGCATGGAGTAA
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACAATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGTCGCC
TGATTCTTAGCGAAGAGTCATGGAATATAGTGATGGATGCCATCAGTAACCCGCCTGCACCGAATGATAGGCTGAAACGA
GCTGCCGAGCGTCTACAAAGCATGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J0QWY8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J0QUA3 |