Detailed information of TA system TA025787

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 1782531..1783953
• Replicon: chromosome
• Accession: NC_016633
• Organism: Sphaerochaeta pleomorpha str. Grapes

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: G8QWE0
• Locus tag: SPIGRAPES_RS08110
• Protein ID: WP_014270286.1
• Coordinates: 1783117..1783953 (+)
• Length: 279 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: G8QWD9
• Locus tag: SPIGRAPES_RS08105
• Protein ID: WP_014270285.1
• Coordinates: 1782531..1783124 (+)
• Length: 198 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPIGRAPES_RS08080	1777827..1778234	-	408	WP_041384524.1	DNA-processing protein DprA	-
SPIGRAPES_RS08085	1778213..1778947	-	735	WP_041384525.1	DNA-processing protein DprA	-
SPIGRAPES_RS08090	1778940..1780478	-	1539	WP_014270283.1	YifB family Mg chelatase-like AAA ATPase	-
SPIGRAPES_RS08095	1780938..1782152	+	1215	WP_014270284.1	ATP-binding protein	-
SPIGRAPES_RS08100	1782261..1782488	+	228	WP_155816686.1	hypothetical protein	-
SPIGRAPES_RS08105	1782531..1783124	+	594	WP_014270285.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
SPIGRAPES_RS08110	1783117..1783953	+	837	WP_014270286.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
SPIGRAPES_RS08115	1783965..1784549	+	585	WP_014270287.1	hypothetical protein	-
SPIGRAPES_RS08120	1784533..1785201	+	669	WP_014270288.1	hypothetical protein	-
SPIGRAPES_RS08125	1785268..1785543	+	276	WP_014270289.1	hypothetical protein	-
SPIGRAPES_RS16565	1785686..1786123	-	438	WP_014270290.1	protein-export chaperone SecB	-
SPIGRAPES_RS08135	1786126..1786443	-	318	WP_014270291.1	hypothetical protein	-
SPIGRAPES_RS08140	1786440..1786850	-	411	WP_014270292.1	hypothetical protein	-
SPIGRAPES_RS08145	1787394..1788758	-	1365	WP_172635078.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-248)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-51)

Sequences

Toxin

Protein

• Length: 279 a.a.
• Molecular weight: 32280.62 Da
• Isoelectric Point: 8.8411

>T25787 WP_014270286.1 NC_016633:1783117-1783953 [Sphaerochaeta pleomorpha str. Grapes]
MNKARLTALCHKISKETGLPFNSVMLYYFLESILKKLVQGKYGGNLVFKGGFLLSNVVGIESRSTMDIDFLLRNMQVSEE
KVLELLTESLKEKEGDEIKYKIMNISPIKEQDQYGGYRASILCRFENIRQVIPLDVATGDVITPHPIQYSFASVFGNDEI
LIKAYPIETMLAEKLQTIYARGFLNSRNKDYYDLHILYRLRSKEIDMSTLARACERTFHNRKTEFDVVKILELLERLKRD
DSFLKHWKVYTKKNNYVQGISFEAVILDAMKLINEMVM

Nucleotide

• Length: 837 bp

>T25787 NC_016633:1783117-1783953 [Sphaerochaeta pleomorpha str. Grapes]
ATGAATAAAGCCAGATTGACTGCACTTTGCCATAAAATCAGCAAGGAAACTGGATTACCCTTTAACTCGGTCATGCTCTA
TTATTTTCTTGAAAGCATATTGAAGAAGCTTGTGCAAGGGAAGTATGGAGGGAATCTTGTTTTCAAAGGTGGCTTTCTAC
TTTCCAATGTCGTAGGAATAGAATCTCGAAGCACTATGGATATAGATTTTCTTTTACGAAACATGCAGGTATCGGAAGAG
AAGGTTTTGGAATTGCTTACTGAATCATTGAAAGAAAAAGAAGGAGATGAAATAAAGTATAAAATTATGAACATCTCACC
AATCAAAGAACAAGATCAATACGGAGGATATCGAGCAAGTATTCTTTGCAGATTTGAAAACATAAGACAAGTCATTCCGC
TTGATGTTGCAACGGGAGATGTTATAACTCCACACCCGATACAATATTCATTTGCCAGTGTCTTTGGGAATGACGAAATC
CTCATTAAGGCATATCCAATTGAAACAATGCTTGCAGAGAAGTTGCAGACAATCTATGCAAGGGGGTTCTTGAATAGCCG
GAATAAGGACTATTATGATCTCCATATCCTTTACAGGTTAAGGAGCAAGGAGATCGATATGTCTACATTGGCAAGAGCCT
GTGAGCGGACATTCCATAATCGTAAAACAGAATTCGATGTAGTAAAAATACTGGAATTGCTGGAAAGATTAAAGCGGGAC
GATTCTTTTCTGAAGCATTGGAAGGTTTATACAAAGAAGAACAACTATGTTCAAGGCATTAGTTTTGAAGCTGTCATCCT
TGATGCTATGAAACTCATTAATGAAATGGTGATGTGA

Antitoxin

Protein

• Length: 198 a.a.
• Molecular weight: 23229.59 Da
• Isoelectric Point: 7.3692

>AT25787 WP_014270285.1 NC_016633:1782531-1783124 [Sphaerochaeta pleomorpha str. Grapes]
MDYKKQIEQHLENSGGIITASYCRSKKIPTVYLSRLVREGDLQRVAEGLYISEEGMYDDYYYFQYRFKKAVFSYETALHL
LGATDKIPQDMDVTVYYSYKFNKAPFGVNIHYVNKEIHGLGVVEARTMFNNPVRVYSYERTLCDFIAHEDKMDREVYVKL
IRSYSKYAKRDIQALYEIATRMGIDKEVKALMEVVYE

Nucleotide

• Length: 594 bp

>AT25787 NC_016633:1782531-1783124 [Sphaerochaeta pleomorpha str. Grapes]
ATGGATTATAAAAAACAAATAGAGCAGCATCTTGAGAACTCAGGTGGAATAATCACGGCATCATACTGCCGGAGCAAAAA
GATTCCAACTGTATATCTTAGCAGGCTCGTTCGAGAAGGTGATCTGCAGAGAGTGGCAGAGGGTCTCTATATTTCGGAAG
AAGGAATGTATGATGACTATTATTATTTTCAATATCGCTTTAAAAAAGCAGTGTTTTCCTATGAGACAGCACTACATCTG
CTGGGGGCAACGGACAAGATTCCTCAAGACATGGATGTTACGGTCTATTATAGCTATAAGTTCAATAAAGCTCCTTTCGG
AGTGAACATCCATTATGTGAATAAGGAAATCCATGGACTTGGAGTGGTGGAAGCTAGAACCATGTTCAATAATCCTGTCA
GGGTTTATTCTTATGAACGAACACTTTGCGATTTTATCGCCCACGAAGATAAAATGGACAGGGAAGTGTATGTCAAGCTT
ATCCGTTCCTATTCAAAATATGCTAAAAGAGATATCCAAGCCCTCTATGAAATAGCAACAAGAATGGGGATTGATAAAGA
GGTAAAAGCACTTATGGAAGTGGTCTATGAATAA

Structures

Toxin

• AlphaFold DB: G8QWE0

Antitoxin

• AlphaFold DB: G8QWD9