Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 787286..787869 Replicon plasmid pHG51
Accession NZ_CP104393
Organism Enterococcus raffinosus strain HG-5

Toxin (Protein)


Gene name mazF Uniprot ID R2S2E9
Locus tag N4S13_RS20100 Protein ID WP_010743953.1
Coordinates 787525..787869 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R2RFE4
Locus tag N4S13_RS20095 Protein ID WP_010743954.1
Coordinates 787286..787531 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S13_RS20065 (N4S13_20065) 782507..783811 + 1305 WP_010743959.1 glycosyltransferase family 2 protein -
N4S13_RS20070 (N4S13_20070) 783938..785023 + 1086 WP_141741229.1 UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) -
N4S13_RS20075 (N4S13_20075) 785111..785203 - 93 WP_111945260.1 type I toxin-antitoxin system Fst family toxin -
N4S13_RS20080 (N4S13_20080) 785285..785902 - 618 WP_010743957.1 DUF2922 family protein -
N4S13_RS20085 (N4S13_20085) 785948..786332 - 385 Protein_740 hypothetical protein -
N4S13_RS20090 (N4S13_20090) 786741..786869 + 129 WP_010743955.1 hypothetical protein -
N4S13_RS20095 (N4S13_20095) 787286..787531 + 246 WP_010743954.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
N4S13_RS20100 (N4S13_20100) 787525..787869 + 345 WP_010743953.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N4S13_RS20105 (N4S13_20105) 788317..788982 + 666 WP_035015986.1 helix-turn-helix domain-containing protein -
N4S13_RS20110 (N4S13_20110) 789476..790948 + 1473 WP_010743951.1 helix-turn-helix domain-containing protein -
N4S13_RS20115 (N4S13_20115) 790965..791744 + 780 WP_010743950.1 AP2 domain-containing protein -
N4S13_RS20120 (N4S13_20120) 791844..792257 + 414 WP_010743949.1 Ohr family peroxiredoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bsh 1..1028198 1028198


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin

SpoVT-AbrB domain

(8-46)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13217.40 Da        Isoelectric Point: 9.2981

>T257860 WP_010743953.1 NZ_CP104393:787525-787869 [Enterococcus raffinosus]
MVRMIKQGDIVKMNLDPKQGHEQKGYRPYICLSYKGVAMNSRIAIFAPISNTQRNYPLYVPVSAECKTTGKVLLDQLVAI
DFYHRDYSFVETVPDDFINDLLKKVKVIFQRDKE

Download         Length: 345 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9154.35 Da        Isoelectric Point: 3.9490

>AT257860 WP_010743954.1 NZ_CP104393:787286-787531 [Enterococcus raffinosus]
MMQIPIGKWGNSNAIRIPVDLLNAIGAKQGDKLNVEIENGKLILGKNVDEELTFEQLFEGYDEEPFDSDLVMFEPTGNEK
W

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R2S2E9

Antitoxin

Source ID Structure
AlphaFold DB R2RFE4

References