Detailed information of TA system TA025786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 5638451..5639106
• Replicon: chromosome
• Accession: NC_016631
• Organism: Granulicella mallensis MP5ACTX8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G8NU58
• Locus tag: ACIX8_RS21825
• Protein ID: WP_014267564.1
• Coordinates: 5638687..5639106 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G8NU57
• Locus tag: ACIX8_RS21820
• Protein ID: WP_014267563.1
• Coordinates: 5638451..5638690 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX8_RS21795	5634993..5635691	-	699	WP_044177273.1	hypothetical protein	-
ACIX8_RS21800	5635887..5636273	-	387	WP_014267559.1	hypothetical protein	-
ACIX8_RS21805	5636419..5637045	-	627	WP_014267560.1	NAD(P)H-dependent oxidoreductase	-
ACIX8_RS21810	5637156..5637728	+	573	WP_014267561.1	TetR/AcrR family transcriptional regulator	-
ACIX8_RS21815	5637742..5638260	-	519	WP_014267562.1	hypothetical protein	-
ACIX8_RS21820	5638451..5638690	+	240	WP_014267563.1	antitoxin	Antitoxin
ACIX8_RS21825	5638687..5639106	+	420	WP_014267564.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACIX8_RS21830	5639409..5640545	-	1137	WP_014267565.1	tRNA guanosine(34) transglycosylase Tgt	-
ACIX8_RS21835	5640966..5641538	-	573	WP_014267566.1	DUF4252 domain-containing protein	-
ACIX8_RS21840	5641561..5641950	-	390	WP_190273719.1	hypothetical protein	-
ACIX8_RS21845	5641937..5642461	-	525	WP_014267568.1	sigma-70 family RNA polymerase sigma factor	-
ACIX8_RS21850	5642732..5643538	-	807	WP_150110710.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14965.25 Da
• Isoelectric Point: 5.6088

>T25786 WP_014267564.1 NC_016631:5638687-5639106 [Granulicella mallensis MP5ACTX8]
MIILDTNVVSEPMRPNGDPRVGAWLDRQMAETLYLTATSLSELLVGVELLPEGKRKQGLASALSDLMNRLFGSRILPFDQ
QAAITYASLIGQARIGGHHLSVADGQIAAIAKVHGFIVATRDTTPFVAAGISILNPWEE

Nucleotide

• Length: 420 bp

>T25786 NC_016631:5638687-5639106 [Granulicella mallensis MP5ACTX8]
ATGATCATCCTGGATACGAATGTTGTATCGGAACCGATGAGGCCCAACGGAGATCCCCGCGTTGGTGCGTGGCTTGACCG
CCAGATGGCCGAAACACTTTATCTGACAGCCACGAGCCTCTCCGAACTGCTGGTCGGAGTTGAACTCCTTCCAGAAGGAA
AGCGGAAGCAAGGGCTTGCCTCTGCGTTAAGCGATTTGATGAACAGGCTCTTCGGATCACGCATCCTACCCTTCGACCAA
CAGGCAGCGATCACCTATGCATCGCTGATCGGCCAAGCGAGAATTGGCGGGCATCATCTCTCTGTCGCTGACGGTCAAAT
AGCCGCCATCGCAAAGGTGCACGGCTTCATCGTGGCGACGCGAGACACAACCCCTTTTGTCGCTGCTGGGATTTCGATTC
TCAATCCGTGGGAAGAATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8789.95 Da
• Isoelectric Point: 5.6958

>AT25786 WP_014267563.1 NC_016631:5638451-5638690 [Granulicella mallensis MP5ACTX8]
MPAVTIRNLSEETHRALKRRAALHGSSTEAEIRAILEEAVRPKTRTMIGTELAAFGQNFEGLQLDLTRDQTPTEPAVFE

Nucleotide

• Length: 240 bp

>AT25786 NC_016631:5638451-5638690 [Granulicella mallensis MP5ACTX8]
ATGCCCGCTGTCACCATCCGCAATCTATCTGAAGAGACGCACCGCGCGCTGAAGCGGCGCGCAGCTCTGCATGGGAGCAG
TACAGAGGCCGAGATCAGGGCCATTCTGGAAGAAGCTGTGCGGCCCAAAACCCGGACCATGATTGGAACGGAACTCGCTG
CGTTTGGCCAGAACTTTGAAGGACTTCAACTGGATCTCACCCGTGATCAGACTCCAACGGAACCGGCAGTGTTTGAATGA

Structures

Toxin

• AlphaFold DB: G8NU58

Antitoxin

• AlphaFold DB: G8NU57