Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3261559..3262192 Replicon chromosome
Accession NZ_CP104390
Organism Weizmannia coagulans strain JBI-YZ6.3

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag N4P52_RS15585 Protein ID WP_013860680.1
Coordinates 3261559..3261909 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag N4P52_RS15590 Protein ID WP_014096808.1
Coordinates 3261914..3262192 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4P52_RS15550 (N4P52_15550) 3256819..3257598 - 780 WP_019720971.1 RNA polymerase sigma factor SigB -
N4P52_RS15555 (N4P52_15555) 3257576..3258046 - 471 WP_168985761.1 anti-sigma B factor RsbW -
N4P52_RS15560 (N4P52_15560) 3258050..3258379 - 330 WP_013860674.1 anti-sigma factor antagonist -
N4P52_RS15565 (N4P52_15565) 3258393..3259460 - 1068 WP_168985762.1 PP2C family protein-serine/threonine phosphatase -
N4P52_RS15570 (N4P52_15570) 3259475..3259876 - 402 WP_013860676.1 anti-sigma regulatory factor -
N4P52_RS15575 (N4P52_15575) 3259881..3260237 - 357 WP_017553290.1 STAS domain-containing protein -
N4P52_RS15580 (N4P52_15580) 3260241..3261068 - 828 WP_168985763.1 RsbT co-antagonist protein RsbRA -
N4P52_RS15585 (N4P52_15585) 3261559..3261909 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N4P52_RS15590 (N4P52_15590) 3261914..3262192 - 279 WP_014096808.1 hypothetical protein Antitoxin
N4P52_RS15595 (N4P52_15595) 3262345..3263496 - 1152 WP_014096807.1 alanine racemase -
N4P52_RS15600 (N4P52_15600) 3263698..3264714 - 1017 WP_014096806.1 outer membrane lipoprotein carrier protein LolA -
N4P52_RS15605 (N4P52_15605) 3264855..3265205 - 351 WP_014096805.1 holo-ACP synthase -
N4P52_RS15610 (N4P52_15610) 3265486..3266085 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T257857 WP_013860680.1 NZ_CP104390:c3261909-3261559 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT257857 WP_014096808.1 NZ_CP104390:c3262192-3261914 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References