Detailed information of TA system TA025785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 5004528..5005163
• Replicon: chromosome
• Accession: NC_016631
• Organism: Granulicella mallensis MP5ACTX8

Toxin (Protein)

• Gene name: tad
• Uniprot ID: G8NP43
• Locus tag: ACIX8_RS19545
• Protein ID: WP_014267113.1
• Coordinates: 5004528..5004851 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: ACIX8_RS19550
• Protein ID: WP_014267114.1
• Coordinates: 5004855..5005163 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX8_RS19530	5000061..5000678	+	618	WP_014267110.1	GNAT family N-acetyltransferase	-
ACIX8_RS19535	5000689..5001840	-	1152	WP_014267111.1	rod shape-determining protein MreC	-
ACIX8_RS19540	5002021..5004465	+	2445	WP_150110678.1	ATP-dependent DNA helicase RecG	-
ACIX8_RS19545	5004528..5004851	+	324	WP_014267113.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACIX8_RS19550	5004855..5005163	+	309	WP_014267114.1	helix-turn-helix domain-containing protein	Antitoxin
ACIX8_RS19555	5005228..5005662	-	435	WP_014267115.1	response regulator	-
ACIX8_RS19560	5006316..5007224	-	909	WP_014267116.1	PhzF family phenazine biosynthesis protein	-
ACIX8_RS19565	5007624..5008280	+	657	WP_014267117.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12182.25 Da
• Isoelectric Point: 9.9943

>T25785 WP_014267113.1 NC_016631:5004528-5004851 [Granulicella mallensis MP5ACTX8]
MGTIKPLEFLGNSRDDLREFPVEVRRAIGAELLRVQLGVMPTDFKPMPAVGKGAYEIRVRLDGAWRVLYVAKFNEAVYVL
HAFQKKTQQTAKEDIDLAAKRYKMIGE

Nucleotide

• Length: 324 bp

>T25785 NC_016631:5004528-5004851 [Granulicella mallensis MP5ACTX8]
ATGGGCACGATCAAACCCCTCGAGTTCCTCGGTAATAGCCGCGATGATCTTCGCGAATTCCCGGTAGAGGTCCGCCGTGC
CATTGGAGCAGAACTACTACGCGTTCAACTGGGCGTCATGCCCACCGATTTCAAGCCAATGCCAGCCGTTGGTAAAGGCG
CCTACGAAATACGTGTCCGTCTCGATGGCGCCTGGCGTGTCCTCTACGTCGCCAAGTTCAATGAAGCTGTTTATGTGCTG
CACGCATTCCAAAAGAAGACACAACAGACCGCCAAAGAAGACATTGATCTCGCGGCCAAACGTTACAAGATGATTGGAGA
ATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11238.03 Da
• Isoelectric Point: 7.2049

>AT25785 WP_014267114.1 NC_016631:5004855-5005163 [Granulicella mallensis MP5ACTX8]
MKKTIPGWDDTRITSSSGNIFVDLGFDEAEAQIMAMRVELMVSIEKHIAAQGWTQAEAAKRLGITQPRVSKLKKGAWDEF
SVDMLLTLAARAGLHPELRLAA

Nucleotide

• Length: 309 bp

>AT25785 NC_016631:5004855-5005163 [Granulicella mallensis MP5ACTX8]
ATGAAAAAGACAATTCCAGGATGGGACGATACTCGAATTACCTCAAGCTCTGGCAACATATTCGTCGATCTGGGGTTCGA
TGAAGCTGAAGCCCAGATCATGGCCATGCGCGTAGAACTCATGGTCAGCATAGAAAAGCACATTGCCGCGCAAGGCTGGA
CACAGGCCGAAGCTGCCAAACGACTGGGGATTACTCAGCCTCGTGTCTCCAAACTCAAAAAGGGAGCCTGGGACGAGTTT
AGTGTGGATATGCTATTGACCTTGGCCGCACGCGCTGGTTTACATCCTGAACTACGCTTGGCAGCCTAG

Structures

Toxin

• AlphaFold DB: G8NP43