Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4110832..4111408 Replicon chromosome
Accession NZ_CP104384
Organism Escherichia coli strain JNQH498

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag KIG00_RS20200 Protein ID WP_001360091.1
Coordinates 4111121..4111408 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KIG00_RS20195 Protein ID WP_000063156.1
Coordinates 4110832..4111134 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIG00_RS20180 (4107428) 4107428..4109578 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KIG00_RS20185 (4109628) 4109628..4109831 + 204 WP_000467859.1 YbdD/YjiX family protein -
KIG00_RS20190 (4109842) 4109842..4110798 + 957 WP_001297640.1 GTPase -
KIG00_RS20195 (4110832) 4110832..4111134 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KIG00_RS20200 (4111121) 4111121..4111408 - 288 WP_001360091.1 BrnT family toxin Toxin
KIG00_RS20205 (4111607) 4111607..4112304 - 698 Protein_3952 IS1 family transposase -
KIG00_RS20210 (4112361) 4112361..4112456 + 96 Protein_3953 dienelactone hydrolase family protein -
KIG00_RS20215 (4112602) 4112602..4113834 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
KIG00_RS20220 (4113875) 4113875..4115155 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T257849 WP_001360091.1 NZ_CP104384:c4111408-4111121 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT257849 WP_000063156.1 NZ_CP104384:c4111134-4110832 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References