Detailed information of TA system TA025783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 2048227..2048811
• Replicon: chromosome
• Accession: NC_016631
• Organism: Granulicella mallensis MP5ACTX8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ACIX8_RS08680
• Protein ID: WP_044176403.1
• Coordinates: 2048524..2048811 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G8NQD6
• Locus tag: ACIX8_RS08675
• Protein ID: WP_014264964.1
• Coordinates: 2048227..2048520 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX8_RS08650	2043719..2044351	-	633	WP_014264959.1	outer membrane lipoprotein carrier protein LolA	-
ACIX8_RS08655	2044455..2044961	-	507	WP_150110539.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ACIX8_RS08660	2045131..2046462	-	1332	WP_014264961.1	insulinase family protein	-
ACIX8_RS08665	2046476..2046907	-	432	WP_014264962.1	DUF4440 domain-containing protein	-
ACIX8_RS08670	2046941..2048167	+	1227	WP_150110540.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACIX8_RS08675	2048227..2048520	+	294	WP_014264964.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ACIX8_RS08680	2048524..2048811	+	288	WP_044176403.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
ACIX8_RS08685	2048815..2049837	-	1023	WP_014264965.1	YvcK family protein	-
ACIX8_RS08690	2049945..2050547	-	603	WP_150110541.1	tetratricopeptide repeat protein	-
ACIX8_RS08695	2050663..2051262	-	600	WP_014264967.1	DUF4254 domain-containing protein	-
ACIX8_RS08700	2051475..2052626	+	1152	WP_014264968.1	glycosyltransferase family 9 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-52)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10903.40 Da
• Isoelectric Point: 4.1473

>T25783 WP_044176403.1 NC_016631:2048524-2048811 [Granulicella mallensis MP5ACTX8]
MAVIWSEAAVQDLQGIFDYVVEDSPQNALLVDTRICEQTDELVRFPALGRPGQFEGTRELIIQRTPYIAIYRVEEDDTIR
VVRILHAAQEWPSVM

Nucleotide

• Length: 288 bp

>T25783 NC_016631:2048524-2048811 [Granulicella mallensis MP5ACTX8]
ATGGCCGTTATCTGGAGTGAAGCTGCTGTTCAGGATCTACAGGGCATCTTCGATTATGTAGTGGAGGACAGTCCGCAGAA
TGCTCTTCTTGTGGATACTCGCATCTGCGAACAGACCGATGAGTTGGTGCGCTTTCCCGCTCTGGGACGACCAGGCCAAT
TTGAAGGCACACGCGAACTGATCATTCAGAGAACTCCATACATCGCCATTTATCGCGTGGAAGAGGATGACACCATCCGA
GTCGTACGCATACTGCATGCAGCGCAGGAGTGGCCTTCTGTGATGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10838.22 Da
• Isoelectric Point: 8.7133

>AT25783 WP_014264964.1 NC_016631:2048227-2048520 [Granulicella mallensis MP5ACTX8]
MAANALVQTRIDADVRDRATAVLEEQGMTVSEAVRILLTRTANEGALPFTPANNEAYNAWFRAKVQEALDDPRPAVSREE
VDRRMAERRASARAKLR

Nucleotide

• Length: 294 bp

>AT25783 NC_016631:2048227-2048520 [Granulicella mallensis MP5ACTX8]
ATGGCCGCTAATGCCTTAGTTCAAACTCGAATCGATGCTGACGTAAGAGATCGCGCTACGGCGGTTCTAGAAGAGCAGGG
TATGACTGTCTCCGAAGCCGTGCGGATTCTTTTGACGCGCACCGCGAACGAAGGGGCTCTGCCTTTTACGCCGGCGAACA
ATGAGGCCTACAACGCCTGGTTTCGTGCCAAAGTGCAGGAGGCGCTGGATGATCCGCGTCCAGCAGTTTCACGGGAAGAG
GTCGATCGGCGAATGGCAGAACGGCGTGCTTCTGCACGAGCCAAATTGCGATAG

Structures

Antitoxin

• AlphaFold DB: G8NQD6