Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5305083..5305708 Replicon chromosome
Accession NZ_CP104373
Organism Klebsiella pneumoniae strain 2021CK-01815

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag N4558_RS25465 Protein ID WP_019705794.1
Coordinates 5305083..5305466 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag N4558_RS25470 Protein ID WP_168234151.1
Coordinates 5305466..5305708 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4558_RS25450 (N4558_25445) 5302449..5303351 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
N4558_RS25455 (N4558_25450) 5303348..5303983 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
N4558_RS25460 (N4558_25455) 5303980..5304909 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
N4558_RS25465 (N4558_25460) 5305083..5305466 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
N4558_RS25470 (N4558_25465) 5305466..5305708 - 243 WP_168234151.1 CopG family transcriptional regulator Antitoxin
N4558_RS25475 (N4558_25470) 5305913..5306830 + 918 WP_019705795.1 alpha/beta hydrolase -
N4558_RS25480 (N4558_25475) 5306844..5307785 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
N4558_RS25485 (N4558_25480) 5307830..5308267 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
N4558_RS25490 (N4558_25485) 5308264..5309124 - 861 WP_004146232.1 virulence factor BrkB family protein -
N4558_RS25495 (N4558_25490) 5309118..5309717 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T257828 WP_019705794.1 NZ_CP104373:c5305466-5305083 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9595.88 Da        Isoelectric Point: 4.3509

>AT257828 WP_168234151.1 NZ_CP104373:c5305708-5305466 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSVLGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure

References