Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4705958..4706768 Replicon chromosome
Accession NZ_CP104373
Organism Klebsiella pneumoniae strain 2021CK-01815

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag N4558_RS22625 Protein ID WP_004178461.1
Coordinates 4705958..4706491 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag N4558_RS22630 Protein ID WP_002887278.1
Coordinates 4706502..4706768 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4558_RS22620 (N4558_22615) 4704789..4705910 + 1122 WP_002887282.1 cupin domain-containing protein -
N4558_RS22625 (N4558_22620) 4705958..4706491 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
N4558_RS22630 (N4558_22625) 4706502..4706768 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
N4558_RS22635 (N4558_22630) 4706871..4708304 - 1434 WP_019705874.1 Cu(+)/Ag(+) sensor histidine kinase -
N4558_RS22640 (N4558_22635) 4708294..4708977 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
N4558_RS22645 (N4558_22640) 4709149..4710534 + 1386 WP_032409188.1 efflux transporter outer membrane subunit -
N4558_RS22650 (N4558_22645) 4710552..4710896 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T257825 WP_004178461.1 NZ_CP104373:c4706491-4705958 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT257825 WP_002887278.1 NZ_CP104373:c4706768-4706502 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References