Detailed information of TA system TA025782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1925656..1926308
• Replicon: chromosome
• Accession: NC_016631
• Organism: Granulicella mallensis MP5ACTX8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G8NNV8
• Locus tag: ACIX8_RS08155
• Protein ID: WP_014264862.1
• Coordinates: 1925922..1926308 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ACIX8_RS08150
• Protein ID: WP_044176374.1
• Coordinates: 1925656..1925922 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX8_RS08130	1920905..1922275	-	1371	WP_083836641.1	MFS transporter	-
ACIX8_RS08135	1922361..1923230	+	870	WP_150110801.1	SCO family protein	-
ACIX8_RS08140	1923265..1923648	+	384	WP_014264859.1	hypothetical protein	-
ACIX8_RS08145	1923731..1925641	+	1911	WP_014264860.1	ABC transporter ATP-binding protein/permease	-
ACIX8_RS08150	1925656..1925922	+	267	WP_044176374.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ACIX8_RS08155	1925922..1926308	+	387	WP_014264862.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACIX8_RS08160	1926305..1928374	-	2070	WP_014264863.1	oligopeptidase B	-
ACIX8_RS08165	1928497..1929015	+	519	WP_014264864.1	DUF4142 domain-containing protein	-
ACIX8_RS08170	1929179..1930036	+	858	WP_052310580.1	TPM domain-containing protein	-
ACIX8_RS08175	1930105..1930689	+	585	WP_014264866.1	LemA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Antitoxin VapB-like (3-85)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14006.31 Da
• Isoelectric Point: 5.1152

>T25782 WP_014264862.1 NC_016631:1925922-1926308 [Granulicella mallensis MP5ACTX8]
MVIDSSALVAILLAEPDASIYISAILNDVTRLISAATLVETSIVMIRRREPDAIAALDAVVARLQLTVIPVDREQALLAR
QGFRRFGKGLDRAGLNFGDCFSYALAMHLNEPLLFKGNDFTFTDVLRA

Nucleotide

• Length: 387 bp

>T25782 NC_016631:1925922-1926308 [Granulicella mallensis MP5ACTX8]
ATGGTTATTGATTCATCAGCATTGGTGGCAATCCTCCTCGCAGAACCTGATGCCTCAATTTATATTTCCGCAATATTGAA
CGATGTGACGAGACTCATCTCTGCTGCGACACTTGTAGAAACCTCTATCGTCATGATCAGAAGAAGAGAGCCTGACGCTA
TTGCCGCGCTCGATGCTGTTGTAGCCCGACTGCAATTGACTGTCATTCCTGTAGATCGTGAACAGGCTCTACTTGCCCGA
CAAGGGTTCCGACGGTTCGGCAAAGGACTGGATCGCGCAGGACTTAATTTCGGTGACTGCTTCTCCTATGCGTTGGCTAT
GCACCTTAACGAGCCCCTGTTGTTCAAGGGAAACGATTTTACGTTCACGGATGTTCTCCGTGCTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9541.80 Da
• Isoelectric Point: 4.2080

>AT25782 WP_044176374.1 NC_016631:1925656-1925922 [Granulicella mallensis MP5ACTX8]
MYMALHITNPAVEQKVRSLASVTGESITEAIGAAAEERLIRFNAAGKNLSSPSVEEVLAMIRSFNLKPINEDLTDDEILG
YGPEGFCE

Nucleotide

• Length: 267 bp

>AT25782 NC_016631:1925656-1925922 [Granulicella mallensis MP5ACTX8]
ATGTACATGGCGTTGCATATTACCAATCCGGCGGTGGAGCAGAAGGTACGAAGCTTGGCCTCTGTTACCGGAGAATCCAT
TACAGAGGCCATTGGTGCGGCCGCGGAAGAGCGCCTGATTCGTTTTAACGCCGCAGGGAAAAACCTATCCTCTCCTTCCG
TTGAAGAGGTTCTGGCGATGATACGGAGCTTCAATCTGAAACCTATCAACGAAGACCTGACGGACGATGAGATTCTGGGG
TACGGCCCAGAAGGTTTTTGCGAGTAA

Structures

Toxin

• AlphaFold DB: G8NNV8