Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3430714..3431211 Replicon chromosome
Accession NZ_CP104371
Organism Escherichia coli strain 2021CK-01784

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N4502_RS16490 Protein ID WP_001173343.1
Coordinates 3431038..3431211 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N4502_RS16485 Protein ID WP_000398619.1
Coordinates 3430714..3431010 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4502_RS16465 (3427443) 3427443..3428162 - 720 WP_000611288.1 two-component system response regulator DcuR -
N4502_RS16470 (3428159) 3428159..3429790 - 1632 WP_001216477.1 sensor histidine kinase -
N4502_RS16475 (3429971) 3429971..3430201 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
N4502_RS16480 (3430213) 3430213..3430485 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N4502_RS16485 (3430714) 3430714..3431010 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N4502_RS16490 (3431038) 3431038..3431211 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N4502_RS16495 (3431329) 3431329..3432846 - 1518 WP_000003806.1 lysine--tRNA ligase -
N4502_RS16500 (3433083) 3433083..3434540 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3430714..3440064 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T257808 WP_001173343.1 NZ_CP104371:3431038-3431211 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT257808 WP_000398619.1 NZ_CP104371:3430714-3431010 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References