Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 684082..684720 Replicon chromosome
Accession NZ_CP104371
Organism Escherichia coli strain 2021CK-01784

Toxin (Protein)


Gene name hicA Uniprot ID D3GRG9
Locus tag N4502_RS03380 Protein ID WP_001314712.1
Coordinates 684544..684720 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag N4502_RS03375 Protein ID WP_077248684.1
Coordinates 684082..684498 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4502_RS03355 (679234) 679234..680175 - 942 WP_001251335.1 ABC transporter permease -
N4502_RS03360 (680176) 680176..681189 - 1014 WP_000220407.1 ABC transporter ATP-binding protein -
N4502_RS03365 (681207) 681207..682352 - 1146 WP_000047443.1 ABC transporter substrate-binding protein -
N4502_RS03370 (682597) 682597..684003 - 1407 WP_000760623.1 PLP-dependent aminotransferase family protein -
N4502_RS03375 (684082) 684082..684498 - 417 WP_077248684.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
N4502_RS03380 (684544) 684544..684720 - 177 WP_001314712.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
N4502_RS03385 (684942) 684942..685172 + 231 WP_000494241.1 YncJ family protein -
N4502_RS03390 (685264) 685264..687225 - 1962 WP_260837354.1 23S rRNA 5-hydroxycytidine C2501 synthase -
N4502_RS03395 (687298) 687298..687834 - 537 WP_000429149.1 DNA-binding transcriptional regulator SutR -
N4502_RS03400 (687926) 687926..689098 + 1173 WP_001236274.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(87-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6746.81 Da        Isoelectric Point: 11.8888

>T257800 WP_001314712.1 NZ_CP104371:c684720-684544 [Escherichia coli]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Download         Length: 177 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15232.60 Da        Isoelectric Point: 4.7286

>AT257800 WP_077248684.1 NZ_CP104371:c684498-684082 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GRG9

Antitoxin

Source ID Structure

References