Detailed information of TA system TA025780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 439019..439584
• Replicon: chromosome
• Accession: NC_016630
• Organism: Filifactor alocis ATCC 35896

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D6GRN6
• Locus tag: HMPREF0389_RS01970
• Protein ID: WP_014262063.1
• Coordinates: 439306..439584 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D6GRN7
• Locus tag: HMPREF0389_RS01965
• Protein ID: WP_014262062.1
• Coordinates: 439019..439306 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMPREF0389_RS01940	434602..435999	-	1398	WP_014262057.1	relaxase/mobilization nuclease domain-containing protein	-
HMPREF0389_RS01945	436074..436355	-	282	WP_014262058.1	hypothetical protein	-
HMPREF0389_RS01950	436425..436766	-	342	WP_014262059.1	mobilization protein	-
HMPREF0389_RS01955	436807..438090	-	1284	WP_169308509.1	antirestriction protein ArdA	-
HMPREF0389_RS01960	438228..438758	-	531	WP_014262061.1	DNA repair protein RadC	-
HMPREF0389_RS09070	438755..438928	-	174	WP_009531054.1	hypothetical protein	-
HMPREF0389_RS01965	439019..439306	+	288	WP_014262062.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HMPREF0389_RS01970	439306..439584	+	279	WP_014262063.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HMPREF0389_RS08985	439618..439779	-	162	WP_156775228.1	hypothetical protein	-
HMPREF0389_RS01975	439779..440465	-	687	WP_014262064.1	DUF4366 domain-containing protein	-
HMPREF0389_RS01980	440455..440694	-	240	WP_014262065.1	DUF4315 family protein	-
HMPREF0389_RS01985	440716..442557	-	1842	WP_014262066.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	394269..474544	80275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-90)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10562.19 Da
• Isoelectric Point: 8.0688

>T25780 WP_014262063.1 NC_016630:439306-439584 [Filifactor alocis ATCC 35896]
MLELVTTGAFRKDLKLAKKRGYDLSLLETVLNTLLEEKTLDQRYHDHALTGNYIGFRECHILPDWLLIYAVNKSQLILTA
SRTGTHSDLFGR

Nucleotide

• Length: 279 bp

>T25780 NC_016630:439306-439584 [Filifactor alocis ATCC 35896]
ATGTTAGAGCTTGTAACCACAGGTGCCTTTCGCAAGGATTTGAAACTTGCCAAAAAGCGAGGCTACGATCTGTCTTTGCT
TGAAACGGTACTAAATACTCTTTTAGAAGAAAAAACCTTGGATCAGCGTTATCACGACCATGCTTTAACCGGAAATTATA
TCGGGTTTCGAGAATGTCATATCCTGCCGGATTGGCTGCTTATTTATGCAGTAAATAAATCACAACTGATCCTCACAGCT
TCAAGGACGGGAACGCATTCCGATTTGTTTGGGAGATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10601.19 Da
• Isoelectric Point: 4.5431

>AT25780 WP_014262062.1 NC_016630:439019-439306 [Filifactor alocis ATCC 35896]
MAKTANINVRIDPETKASAEQLFSSFGITVTDAINIFLHKSLMEGGLPFEMKQPRFNLETEMAMEEAKLIMAGKIPAKRY
ASVHELLDELETDGE

Nucleotide

• Length: 288 bp

>AT25780 NC_016630:439019-439306 [Filifactor alocis ATCC 35896]
ATGGCAAAAACGGCGAATATCAATGTACGTATTGATCCGGAAACAAAAGCAAGTGCGGAACAGTTATTTTCAAGCTTTGG
AATTACCGTAACCGATGCCATCAATATTTTTCTTCATAAATCACTGATGGAGGGAGGGCTTCCTTTTGAAATGAAACAGC
CTCGTTTCAACTTGGAAACGGAAATGGCGATGGAGGAAGCAAAGCTCATTATGGCAGGAAAAATTCCCGCCAAGCGTTAT
GCCTCGGTACACGAGTTATTGGATGAGCTGGAAACGGATGGTGAATAA

Structures

Toxin

• AlphaFold DB: D6GRN6

Antitoxin

• AlphaFold DB: D6GRN7