Detailed information of TA system TA025772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-PHA01976
• Location: 444337..444965
• Replicon: plasmid AZO_p3
• Accession: NC_016623
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G7ZEM5
• Locus tag: AZOLI_RS23420
• Protein ID: WP_014249676.1
• Coordinates: 444337..444588 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G7ZEM6
• Locus tag: AZOLI_RS23425
• Protein ID: WP_014249677.1
• Coordinates: 444585..444965 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS32705	440158..440607	-	450	Protein_358	hypothetical protein	-
AZOLI_RS23400	440768..441283	+	516	WP_014249673.1	recombinase family protein	-
AZOLI_RS31630	441570..441914	+	345	WP_162488390.1	DUF4145 domain-containing protein	-
AZOLI_RS23405	441953..442774	+	822	WP_012976163.1	IS5-like element ISAli1 family transposase	-
AZOLI_RS23420	444337..444588	+	252	WP_014249676.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZOLI_RS23425	444585..444965	+	381	WP_014249677.1	helix-turn-helix transcriptional regulator	Antitoxin
AZOLI_RS23430	445199..448720	+	3522	WP_162488410.1	indolepyruvate ferredoxin oxidoreductase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..648491	648491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (21-82)

Antitoxin

• Cro/C1-type helix-turn-helix domain (72-123)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9201.81 Da
• Isoelectric Point: 10.6919

>T25772 WP_014249676.1 NC_016623:444337-444588 [Azospirillum lipoferum 4B]
MKLVIEATALKVLLRMPKGDAAGLRDKLKTFAAAPYDPHPWAKAFGGGQGRIRHGDWRAIYQIDGDALLVTVLKVGNRKE
VYR

Nucleotide

• Length: 252 bp

>T25772 NC_016623:444337-444588 [Azospirillum lipoferum 4B]
ATGAAGCTCGTGATCGAAGCCACTGCGCTGAAGGTTCTGCTCCGCATGCCAAAGGGCGATGCGGCCGGGCTGCGGGACAA
GCTGAAGACCTTCGCCGCCGCTCCTTATGACCCCCATCCCTGGGCGAAGGCGTTCGGCGGCGGGCAAGGCCGCATCCGCC
ACGGGGACTGGCGGGCCATCTACCAGATCGACGGCGATGCGCTGTTGGTCACGGTGCTGAAGGTCGGGAACCGCAAGGAG
GTCTATCGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13318.04 Da
• Isoelectric Point: 4.3319

>AT25772 WP_014249677.1 NC_016623:444585-444965 [Azospirillum lipoferum 4B]
MNALPITPIAETADSVTLSRADFEALADLVTDAQDLADAETVKARLAAGETETFPFEVAERILDGEHPVAVLREHRGLTA
RGLAEAAGVAPSYLSEIENGKKPGSFDAMARIAGALQVPLDLLVRH

Nucleotide

• Length: 381 bp

>AT25772 NC_016623:444585-444965 [Azospirillum lipoferum 4B]
ATGAACGCTCTGCCAATCACGCCGATTGCCGAGACGGCGGACAGCGTCACCCTCAGCCGGGCCGACTTCGAGGCCCTGGC
CGATCTGGTGACGGACGCCCAGGACTTGGCCGACGCCGAAACCGTAAAGGCACGGCTCGCTGCGGGGGAGACGGAGACTT
TTCCGTTCGAGGTCGCGGAGCGCATCCTTGATGGGGAGCATCCTGTTGCGGTGCTCCGCGAGCATCGCGGCCTCACGGCC
CGCGGACTGGCGGAAGCCGCAGGCGTGGCTCCGTCCTACCTGTCCGAGATTGAGAACGGGAAAAAGCCCGGCAGCTTCGA
CGCGATGGCCCGCATCGCCGGTGCGCTCCAGGTGCCGCTGGACCTGCTGGTCCGCCACTGA

Structures

Toxin

• AlphaFold DB: G7ZEM5

Antitoxin

• AlphaFold DB: G7ZEM6