Detailed information of TA system TA257714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2221660..2223485
• Replicon: chromosome
• Accession: NZ_CP104354
• Organism: Vibrio cholerae strain PNUSAV001140

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: N4267_RS10060
• Protein ID: WP_260813060.1
• Coordinates: 2221660..2223021 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: N4267_RS10065
• Protein ID: WP_032472658.1
• Coordinates: 2223018..2223485 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N4267_RS10035 (N4267_10035)	2217133..2217579	+	447	WP_000065400.1	DUF2787 domain-containing protein	-
N4267_RS10040 (N4267_10040)	2217682..2218668	+	987	WP_000858603.1	GTPase family protein	-
N4267_RS10045 (N4267_10045)	2218703..2219344	-	642	WP_069647815.1	inovirus Gp2 family protein	-
N4267_RS10050 (N4267_10050)	2219945..2220742	+	798	WP_260813059.1	CopG family transcriptional regulator	-
N4267_RS10055 (N4267_10055)	2220822..2221022	-	201	WP_001114737.1	AlpA family transcriptional regulator	-
N4267_RS10060 (N4267_10060)	2221660..2223021	+	1362	WP_260813060.1	type II toxin-antitoxin system HipA family toxin	Toxin
N4267_RS10065 (N4267_10065)	2223018..2223485	+	468	WP_032472658.1	helix-turn-helix transcriptional regulator	Antitoxin
N4267_RS10070 (N4267_10070)	2223913..2224491	+	579	WP_134986936.1	class I SAM-dependent methyltransferase	-
N4267_RS10075 (N4267_10075)	2224495..2227443	+	2949	WP_134986935.1	DEAD/DEAH box helicase family protein	-
N4267_RS10080 (N4267_10080)	2227514..2228215	+	702	Protein_1959	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2210640..2229062	18422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-384)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51241.13 Da
• Isoelectric Point: 4.9109

>T257714 WP_260813060.1 NZ_CP104354:2221660-2223021 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEVLIKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17073.60 Da
• Isoelectric Point: 10.7084

>AT257714 WP_032472658.1 NZ_CP104354:2223018-2223485 [Vibrio cholerae]
MKPSNKESVQDTVARLRANRNQQQPKVGKNATHSIKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp