Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1709572..1710356 Replicon chromosome
Accession NC_016622
Organism Azospirillum lipoferum 4B

Toxin (Protein)


Gene name TacT2 Uniprot ID G7Z7Z0
Locus tag AZOLI_RS07935 Protein ID WP_014248090.1
Coordinates 1709865..1710356 (+) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID G7Z1L1
Locus tag AZOLI_RS07930 Protein ID WP_014248089.1
Coordinates 1709572..1709868 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZOLI_RS07915 1706801..1707619 + 819 WP_014248086.1 enoyl-CoA hydratase -
AZOLI_RS07920 1707616..1708137 + 522 WP_014248087.1 CoA-binding protein -
AZOLI_RS07925 1708159..1709454 + 1296 WP_014248088.1 O-acetylhomoserine aminocarboxypropyltransferase -
AZOLI_RS07930 1709572..1709868 + 297 WP_014248089.1 DUF1778 domain-containing protein Antitoxin
AZOLI_RS07935 1709865..1710356 + 492 WP_014248090.1 GNAT family N-acetyltransferase Toxin
AZOLI_RS07940 1710363..1711604 - 1242 WP_014248091.1 MFS transporter -
AZOLI_RS07945 1711739..1712158 - 420 WP_014248092.1 aminoacyl-tRNA hydrolase -
AZOLI_RS07950 1712155..1713057 - 903 WP_014248093.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AZOLI_RS07955 1713114..1713422 - 309 WP_014248094.1 AzlD domain-containing protein -
AZOLI_RS07960 1713415..1714149 - 735 WP_044549864.1 AzlC family ABC transporter permease -
AZOLI_RS07965 1714160..1715044 - 885 WP_014248096.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-141)

Antitoxin

Vibrio phage ICP1, Orf50

(16-90)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17526.25 Da        Isoelectric Point: 9.0219

>T25769 WP_014248090.1 NC_016622:1709865-1710356 [Azospirillum lipoferum 4B]
MTLSAPEPLASHHRVDLFESGTPSLDNWLKRRAIANQASGASRTFVACAGDEIAGYYALASSAVAVAGAPERFRRNMPDP
IPVVVLARLAVSRTRQGQGLGRALFRDAALRVVQAADLIGVRGLIVHALSEEAKGFYLRLGLDTSPMDPMMLMVTLAELR
DNL

Download         Length: 492 bp

>T25769 NC_016622:1709865-1710356 [Azospirillum lipoferum 4B]
ATGACGCTGTCCGCGCCCGAGCCGCTTGCATCCCACCATCGCGTCGACCTGTTCGAGTCCGGCACGCCGTCCTTGGACAA
TTGGTTGAAACGCCGCGCCATCGCCAATCAGGCCAGCGGCGCATCACGGACATTCGTCGCCTGCGCCGGGGACGAGATCG
CCGGCTATTACGCTCTGGCATCGAGTGCGGTCGCGGTTGCCGGTGCGCCGGAACGCTTCCGCCGCAACATGCCCGATCCG
ATCCCGGTGGTGGTGCTGGCGCGGCTCGCCGTTTCCCGGACACGGCAGGGGCAGGGCCTCGGCCGGGCGCTGTTCCGTGA
CGCCGCCCTGCGGGTGGTGCAGGCCGCCGATCTGATCGGCGTCCGCGGGCTGATTGTCCATGCGCTGTCGGAAGAGGCGA
AGGGGTTTTACCTTCGCCTCGGCCTCGATACCTCGCCGATGGACCCGATGATGCTGATGGTCACGCTGGCCGAGCTGCGG
GACAATCTGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11105.55 Da        Isoelectric Point: 8.6575

>AT25769 WP_014248089.1 NC_016622:1709572-1709868 [Azospirillum lipoferum 4B]
MQPVEPSEEKSRRETLNIRIRPEERSLIDRAAQAQGKNRTDFILEAARRAAEDALLDRAIIAASPEAYAAFLARLDAPPK
PNEKLRRTLRTPAPWDSE

Download         Length: 297 bp

>AT25769 NC_016622:1709572-1709868 [Azospirillum lipoferum 4B]
ATGCAGCCTGTCGAACCGTCAGAAGAGAAGTCCCGGCGCGAGACGCTGAACATCCGCATCCGCCCGGAAGAGCGCAGCCT
GATCGACCGGGCGGCCCAGGCGCAGGGCAAGAACCGCACGGATTTCATCCTGGAGGCCGCCCGCCGCGCCGCCGAGGATG
CTCTGCTCGACCGCGCGATCATCGCGGCCAGCCCCGAGGCCTACGCCGCGTTCCTCGCCCGGCTCGACGCGCCGCCCAAG
CCGAACGAAAAGCTGCGCCGGACCTTGCGGACACCGGCACCCTGGGACAGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G7Z7Z0

Antitoxin

Source ID Structure
AlphaFold DB A0A5A9FNC3

References