Detailed information of TA system TA025768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1684059..1684618
• Replicon: chromosome
• Accession: NC_016622
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: G7Z5H5
• Locus tag: AZOLI_RS07810
• Protein ID: WP_014248063.1
• Coordinates: 1684059..1684313 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: G7Z1J1
• Locus tag: AZOLI_RS07815
• Protein ID: WP_014248064.1
• Coordinates: 1684310..1684618 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS07780	1679468..1680901	-	1434	WP_014248055.1	AAA family ATPase	-
AZOLI_RS07785	1680898..1681107	-	210	WP_014248056.1	hypothetical protein	-
AZOLI_RS07790	1681100..1681381	-	282	WP_044549848.1	hypothetical protein	-
AZOLI_RS07795	1681378..1681668	-	291	WP_044549851.1	hypothetical protein	-
AZOLI_RS07800	1681668..1682135	-	468	WP_162488017.1	hypothetical protein	-
AZOLI_RS32365	1682183..1682422	-	240	WP_162488018.1	hypothetical protein	-
AZOLI_RS32370	1682425..1682619	-	195	WP_162488019.1	hypothetical protein	-
AZOLI_RS32375	1682756..1683616	+	861	WP_162488020.1	hypothetical protein	-
AZOLI_RS07810	1684059..1684313	+	255	WP_014248063.1	BrnT family toxin	Toxin
AZOLI_RS07815	1684310..1684618	+	309	WP_014248064.1	BrnA antitoxin family protein	Antitoxin
AZOLI_RS07820	1684669..1684926	+	258	WP_014248065.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AZOLI_RS07825	1685000..1686118	+	1119	WP_014248066.1	site-specific integrase	-
AZOLI_RS32380	1686445..1686600	-	156	WP_014248067.1	hypothetical protein	-
AZOLI_RS31040	1686671..1686805	-	135	WP_014248068.1	DUF1289 domain-containing protein	-
AZOLI_RS07830	1687010..1688155	+	1146	WP_014248069.1	ATP-grasp domain-containing protein	-
AZOLI_RS07835	1688343..1688762	-	420	WP_012974451.1	RNA polymerase-binding protein DksA	-
AZOLI_RS07840	1688952..1689377	-	426	WP_014248070.1	flagellar assembly protein FliX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-99)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9312.97 Da
• Isoelectric Point: 8.6190

>T25768 WP_014248063.1 NC_016622:1684059-1684313 [Azospirillum lipoferum 4B]
MKIVYDPPKRITNLDKHGLDFADLEDGAFFETALVIPSRDGRFKAVGWLGDVLVVVVVFKPLGTEALSVISMRPASIKER
KLLT

Nucleotide

• Length: 255 bp

>T25768 NC_016622:1684059-1684313 [Azospirillum lipoferum 4B]
ATGAAGATCGTCTACGACCCTCCGAAGCGCATCACCAACCTGGACAAGCACGGGCTGGACTTTGCCGATCTGGAGGACGG
GGCGTTCTTCGAAACCGCCTTGGTCATCCCGTCGCGCGATGGCCGCTTCAAGGCCGTGGGCTGGCTGGGCGACGTGCTGG
TGGTCGTCGTGGTGTTCAAGCCGCTGGGAACGGAGGCGCTCTCCGTCATCAGCATGCGCCCGGCAAGCATCAAGGAAAGG
AAGCTCCTGACATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11769.21 Da
• Isoelectric Point: 5.0009

>AT25768 WP_014248064.1 NC_016622:1684310-1684618 [Azospirillum lipoferum 4B]
MTEDRKLLTEFQPGRGYSKEDWDEVSDNPELTEEEMRQARPFAEVFPQLAESLKRGRGRPPVEKPKQQVTLRLDADVIER
FKAGGPGWQSRINDELRKSVGL

Nucleotide

• Length: 309 bp

>AT25768 NC_016622:1684310-1684618 [Azospirillum lipoferum 4B]
ATGACCGAAGACCGGAAGCTGCTGACCGAATTTCAGCCGGGACGCGGATACAGCAAAGAGGACTGGGACGAGGTGTCCGA
CAACCCGGAACTGACCGAGGAGGAAATGCGGCAGGCCCGCCCCTTCGCCGAAGTGTTCCCGCAGCTGGCGGAGAGCCTGA
AGCGCGGCCGGGGACGTCCGCCGGTGGAGAAGCCCAAGCAGCAGGTGACGCTGCGCCTGGACGCCGATGTGATCGAGCGC
TTCAAGGCCGGCGGGCCGGGCTGGCAGTCCCGCATCAACGACGAACTGCGCAAGTCCGTGGGGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A5A9FLG2

Antitoxin

• AlphaFold DB: G7Z1J1